壬基酚溶液暴露对秀丽隐杆线虫的毒性研究  

Study of toxicity of Caenorhabditis elegans exposed to nonylphenol solution

作　　者：李安 潘立刚 张秀彤 梁刚 满燕 靳欣欣 LI An;PAN Li-gang;ZHANG Xiu-tong;HANG Gang;MAN Yan;JIN Xin-xin(Beijing Research Center for Agricultural Standards and Testing, Beijing 100097, China;Risk Assessment Lab of Agri-products Quality and Safety of Ministry of Agrieulture（Beijing）, Beijing 100097, China)

机构地区：[1]北京农业质量标准与检测技术研究中心,北京100097 [2]农业部农产品质量安全风险评估实验室(北京),北京100097

出　　处：《农业环境科学学报》2017年第12期2414-2420,共7页Journal of Agro-Environment Science

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20150408)~~

摘　　要：为探究壬基酚溶液暴露对秀丽隐杆线虫的急性致死毒性和非致死毒性效应,将同期化后的秀丽隐杆线虫暴露于壬基酚溶液24 h,考察壬基酚对秀丽隐杆线虫的半致死浓度、性腺臂发育、子代数目、虫体长度和身体弯曲频率等毒性指标的影响。结果表明,以L4期野生型秀丽隐杆线虫为受试生物,对壬基酚溶液24 h的半数致死浓度即LC50值为8.09 mg·L^(-1)。壬基酚暴露干扰了秀丽隐杆线虫性腺臂的正常发育,与对照组相比,0.081 mg·L^(-1)和0.162 mg·L^(-1)染毒组对秀丽隐杆线虫的子代数目和虫体长度均有显著性影响(P<0.05);低剂量染毒组身体弯曲频率与对照组相比没有统计学差异(P>0.05),而高剂量组与对照组有显著性差异(P<0.05)。毒性试验研究表明,模式动物秀丽隐杆线虫对壬基酚的毒性响应比较敏感,在环境激素的生态毒理学研究中具有潜在的应用价值。To better understand the effect of acute lethal toxicity and non-lethal toxicity on Caenorhabditis elegans exposed to nonylphenol solution, C. elegans were exposed to nonylphenol solution for 24 h and then toxicity indicators such as lethal concentration, development of a gonad arm, number of offspring, body length, and body bending frequency were investigated. Results showed that the median lethal concen- tration LC50 for 24 h acute exposure was 8.09 mg·L-1 when using L4 wild-type C. elegans as the testing animal. Nonylphenol can interfere with the normal development of the gonad arm of C. elegans. Compared with the control, both the 0.081 mg ·L-1 and 0.162 mg·L-1 of nonylphenol treatment had significant effects on the number of offspring in C. elegans and the body length of the worm（P〈0.05 ）. The body bending frequency of C. e legans exposed to low doses of nonylphenol showed no statistical difference compared with the control（P〉0.05 ）, whereas high dose treatment showed significant difference （P〈0.05）. The results of the toxicity experiments indicate that the model animal C. elegans has certain sensitivity to nonylphenol. Thus, using C. elegans as a model animal has potential application value in the ecological toxicology of environmental hormones.

关 键 词：壬基酚 秀丽隐杆线虫 生态毒理 

分 类 号：X503.22[环境科学与工程—环境工程]