大丽轮枝菌黑色素合成相关基因与微菌核形成的关系  

The role of DHN melanin biosynthesis genes in microsclerotium formation in Verticillium dahliae

作　　者：樊荣 徐小鸿 曹亚松 商文静[1] 朱荷琴[2] 胡小平[1] 

机构地区：[1]西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]中国农业科学院棉花研究所,河南安阳455000

出　　处：《菌物学报》2017年第12期1608-1615,共8页Mycosystema

基　　金：国家自然科学基金(31371888)~~

摘　　要：大丽轮枝菌是一种重要的土传植物病原真菌,以休眠结构微菌核作为初始接种体,可侵染660多种植物引致黄萎病。微菌核是致密的多细胞结构,表面附着大量的DHN黑色素。许多报道指出,在微菌核发育过程中,传统的DHN黑色素合成途径中有5种催化酶编码基因Vd PKS、Vd T4HR、Vd SCD、Vd T3HR和Vd LAC均被诱导表达,但这些基因与微菌核形成的关系目前尚无报道。本研究通过基因敲除技术,系统研究了传统DHN黑色素合成通路上这5种关键酶编码基因及一种缩链催化酶编码基因Vayg1在大丽轮枝菌黑色素合成及微菌核形成中的作用。结果表明,大丽轮枝菌DHN黑色素合成需要Vayg1基因的参与,且Vayg1和Vd T3HR基因还参与微菌核的形成过程。因此,Vayg1基因和Vd T3HR基因可作为黄萎病防治的新靶标。The soil-born plant fungal pathogen Ve rticilliurn dahliae, with its resting structure of microsclerotia as primary an 660 plant species. The well-developed microsclerotia are thick-walled ;ited. Many studies suggest that expressions of five genes, VdPKS, VdT4HR, involved in DHN melanin biosynthesis were induced during microsclerotium n formation remains unclear. In the current study, melanin deficient mutants /aygl gene, which is responsible for chain-length shortening of a precursor,were used to systemically elucidate their role on melanin production and microsclerotium formation in V. dahliae. The results showed that Vaygl is required for DHN melanin production in V. dahlioe. Vaygl and VdT3HR are involved in microsclerotium formation, indicating that these two enzymes would be potential targets for Verticillium wilt management

关 键 词：DHN黑色素 Vayg1 VdT3HR 微菌核 黄萎病防治靶标 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]