阿奇霉素对铜绿假单胞菌I类整合酶基因表达的影响  

The effect of azithromycin on the class 1 integrase intI1 gene expression in Pseudomonas aeruginosa

作　　者：朱艮苗[1,2] 吴红霞 杨维青 陈萍 刘锋[1] 黄娟[2] 

机构地区：[1]重庆市巴南区人民医院检验科,重庆401320 [2]广东医科大学检验学院,东莞523808

出　　处：《中国抗生素杂志》2017年第11期1003-1006,共4页Chinese Journal of Antibiotics

基　　金：解放军总后卫生部项目(No.CNSC-J2011-A330-ZA028);重庆市集成示范计划项目(No.cstc2015jcsf10002)

摘　　要：目的通过研究阿奇霉素对铜绿假单胞菌I类整合子整合酶基因int I1表达的影响,初步探讨阿奇霉素对I类整合子的抑制机制。方法采用荧光定量RT-PCR方法检测铜绿假单胞菌I类整合酶基因int I1在不同阿奇霉素浓度中m RNA的表达水平,分析阿奇霉素对int I1表达的影响。结果阿奇霉素对铜绿假单胞菌I类整合酶基因int I1的表达抑制作用明显,I类整合酶基因int I1m RNA随着阿奇霉素浓度的增高表达水平呈明显下降趋势。结论阿奇霉素对I类整合酶基因int I1表达具有明显抑制作用,提示可能与阿奇霉素对铜绿假单胞菌群体感应系统抑制机制间接相关。Objective The mechanism of azithromycin inhibition on the class 1 integron were preliminarily explored by studying the influence of azithromycin on the class 1 integron integrase intI1 gene expression in Pseudomonas aeruginosa.Methods Using fluorescence quantitative RT-PCR to detect class 1 integrase intI1 gene of Pseudomonas aeruginosa mRNA expression level in different concentrations of azithromycin,analysis of azithromycin influence on the intI1 expression.Results Azithromycin had a significant inhibitory effect on the expression of the class 1 integrase intI1 gene of Pseudomonas aeruginosa,then the class 1 integrase intI1 gene mRNA expression level increased as the concentration of azithromycin was significantly lower than the half lethal dose （2μg/mL）.And the growth of PA was significantly suppressed in AZM 4μtg/mL medium.Conclusion Azithromycin has an obvious inhibitory on the expression of the class 1 integrase intI1 gene,which suggests it may be associated with azithromycin inhibition on the quorum-sensing system ofPseudomonas aeruginosa indirectly.

关 键 词：铜绿假单胞菌 阿奇霉素 Ⅰ类整合子 整合酶基因 逆转录PCR 

分 类 号：R978.15[医药卫生—药品]