手性流动相添加剂法拆分辛弗林对映体  

Resolution of Synephrine Enantiomeric Using Chiral Mobile Phase Additive

作　　者：翟明翚[1] 于浩[1] 刘呈利 孟培[1] 苏立强[1] ZHAI Ming-hui;YU Hao;LIU Cheng-li;MENG Pei;SU Li-qiang(College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化学试剂》2017年第12期1344-1346,1360,共4页Chemical Reagents

基　　金：黑龙江省教育厅资助项目(12531757)

摘　　要：建立了高效液相色谱手性流动相添加剂法拆分辛弗林对映体的方法。选用C18色谱柱,以0.5 g/L羧甲基-β-环糊精(CM-β-CD)为添加剂,流动相为混合液(V(乙腈)∶V(甲醇)∶V(水)=70∶15∶15),紫外检测波长为202 nm,流量为0.2m L/min,pH 4.20。在此条件下,辛弗林对映体分离度为1.47。The enantiomeric separation of Synephrine by HPLC with chiral mobile phase additive was established.The C18 column was used as stationary phase,using the 0. 5 g/L carboxymethyl-β-cyclodextrin（CM-β-CD） as chiral mobile phase additive,and a mixture of acetonitrile-methanol-water（mixed in the ratio of 70 ∶ 15 ∶ 15 by volume） was used as mobile phase,the UV detection wavelength was set at 202 nm,at a flow-rate of 0. 2 m L/min,pH 4. 20.Under the optimized conditions,the resolution of Synephrine enantiomer was 1. 47.

关 键 词：高效液相色谱 手性流动相添加剂 拆分 辛弗林对映体 

分 类 号：O657.7[理学—分析化学]