银莱汤对发热大鼠血清IL-1β、TNF-α、IL-6含量的影响  

Effect of Yinlai Decoction on IL-1β,TNF-α,IL-6 Levels in Serum of Fever Rats

作　　者：万宇翔[1] 郑子安[1] 白辰 王云辉[1] 于河[1] 刘铁钢[1] 谷晓红[1] WAN Yuxiang;ZHENG Zian;BAI Chert;WANG Yunhui;YU He;LIU Tiegang;GU Xiaohong(School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《湖南中医药大学学报》2017年第11期1184-1187,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(81403328);北京市教委产学研项目(1000062520040);北京中医药大学自主选题资助项目(2016-JYBXS031)

摘　　要：目的研究银莱汤对发热大鼠体温和血清IL-1β、TNF-α、IL-6含量的影响。方法 90只SD大鼠连续测温3次,选取合格大鼠背部皮下注射20%干酵母混悬液10 m L/kg建立大鼠发热模型,随机平均分为正常组、模型组、阿司匹林组、银莱汤高剂量组、银莱汤中剂量组、银莱汤低剂量组共6组,每组8只大鼠。分别于造模前0.5 h和造模后5 h两次灌胃给药,造模后每小时测温一次,于末次给药后3 h取血,通过ELISA和RIA方法检测血清中炎性介质白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子α(TNF-α)含量水平。结果银莱汤各剂量组体温均低于模型组(P<0.01),银莱汤各剂量组血清中IL-1β、TNF-α、IL-6含量均低于模型组(P<0.05)。结论银莱汤对发热大鼠具有明显的退热效果,其作用机制可能与降低机体炎症反应,减少血清中IL-1β、TNF-α、IL-6的产生或加速降解相关。Objective To explore the effect of Yinlai decoction on body temperature,Serum Content of IL-1β,TNF-α,IL-6 of Fever Rats.Methods 90 male rats were randomly divided into normal group,model group,aspirin group,and high,middle and low dose Yinlai decoction groups,8 rats in each group.Fever rat models were induced with dorsal hypodermic injection of 20% dry yeast suspension,and they were fed with leechdom half an hour before building the fever model and 5 hours after building the fever model.The blood were taken at the 8th hour after building the model.The levels of IL-6,IL-1β and TNF-α in serum were detected by ELISA and RIA.Results Temperature in different doses of Yinlai decoction groups are lower than the model group（P〈0.01）.The IL-1β,TNF-α,IL-6 levels in serum were significantly lower than those in the model group（P〈0.05）.Conclusion Yinlai decoction shows obvious anti-febrile effect on fever rats.Its mechanism may relate to restraining the inflammation reaction and reducing the expression of IL-1β,TNF-α,IL-6 in serum.

关 键 词：银莱汤 发热 炎症 IL-1Β TNF-Α IL-6 

分 类 号：R289.5[医药卫生—方剂学] R392.7[医药卫生—中药学]