羟基红花黄色素A对雌激素效应相关蛋白表达的影响  

Influence of Hydroxysafflor Yellow A on Related Protein Expression of Estrogenic Effects

作　　者：陈梦 赵丕文 孙丽萍 武虹波 赵笛 CHEN Meng;ZHAO Piwen;SUN Liping;WU Hongbo;ZHAO Di(School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学中医学院,北京100029

出　　处：《湖南中医药大学学报》2017年第11期1218-1221,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学青年基金(81673764);北京中医药大学自主科研课题(2017-JYB-JS-001)

摘　　要：目的探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对雌激素效应相关蛋白表达的影响。方法培养雌激素受体阳性细胞T47D和特异性雌激素受体(estrogen receptor,ER)阴性细胞SK-BR-3,将1×10^(-7)mol/L^(-1)×10^(-9)mol/L三种梯度浓度的HSYA作用于两种细胞,通过蛋白质免疫印迹法检测雌激素受体ERα及ERβ、细胞外调解蛋白激酶1/2(ERK1/2)及p-ERK1/2、孕激素受体(progesterone receptor,PR)、抑癌基因P27等蛋白的表达情况。结果与空白对照组相比,在T47D细胞中,高浓度的HSYA促进了ERα、ERβ、ERK1/2、p-ERK1/2、PR的表达,抑制了P27的表达(P<0.01);在SK-BR-3细胞中,HSYA显著抑制了ERK1/2的表达,但对p-ERK1/2的表达影响不明显(P>0.05)。结论 HSYA在细胞内影响雌激素效应相关蛋白的表达情况不同,可能是通过调节ER表达及激活ERK信号通路而发挥雌激素样作用。Objective To explore the effect of hydroxylsafflor yellow A（HSYA） on related protein of estrogenic effect.Methods The positive cells T47D and ER,and negative cells SK-BR-3 of estrogen receptor（ER） were cultured,and 1×10^-7 mol/L×10^-9 mol/L of HSYA act on this two cells.,negative cells SK-BR-3.The expression of protein ERα,ERβ,extracellular regulated protein kinases 1/2（ERK1/2）,p-ERK1/2,Progesterone Receptor（PR）,P27 was detected by Western Blot.Results Compared with the blank control group,the high concentration HSYA promoted the expression of Erα,ERβ,ERK1/2,p-ERK1/2,PR,while inhibite the expression of P27 in T47D cells（P 0.01）.HSYA showed significant inhibitory effects on ERK1/2,while had no obvious effects on p-ERK1/2 in SK-BR-3 cells（P〈0.05）.Conclusion HSYA has different influnces on related protein of estrogenic effect in the cells,which possiblly through regulating the expression of ER and activating the ERK signaling pathway.

关 键 词：羟基红花黄色素A WESTERN BLOT T47D细胞 SK-BR-3细胞 

分 类 号：R285.5[医药卫生—中药学] R393[医药卫生—中医学]