麦冬提取物对胰岛素抵抗大鼠肝组织miRNA-29a及FOXO3表达的影响  

Effect of Ophiopogonis Radix Extract on Expressions of miRNA-29a and FOXO3 in Liver Tissues of Insulin Resistant Rats

作　　者：王磊[1] 秦玖刚[2] 王川[2] WANG Lei;QIN Jiu-gang;WANG Chuan(The First Affiliated Hospital of Nanyang Medical College, Nanyang 473000, China;Department of Clinical Medicine, Nanyang Medical College, Nanyang 473000, China)

机构地区：[1]南阳医学高等专科学校第一附属医院,河南南阳473000 [2]南阳医学高等专科学校临床医学系,河南南阳473000

出　　处：《中国实验方剂学杂志》2018年第1期124-129,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省医学科技攻关计划项目(201503046)

摘　　要：目的：探讨麦冬提取物对胰岛素抵抗大鼠肝组织中miRNA-29a及FOXO3表达的影响。方法：选取60只SD级雄性大鼠,随机选取10只为正常组,其余50只建立胰岛素抵抗大鼠模型,造模成功后分为模型组、麦冬提取物高、中、低剂量组、二甲双胍组5组,每组10只,麦冬提取物高、中、低剂量组分别给予150,300,500 mg·kg^-1麦冬提取物灌胃给药干预,二甲双胍组给予200 mg·kg^-1二甲双胍混悬液灌胃给药干预,正常组及模型组给予等体积的生理盐水,分别于给药4,8周后,检测各组大鼠体重（BW）,肝重（LW）,空腹血糖（FBG）,血清总胆固醇（TC）,甘油三酯（TG）,空腹胰岛素（FINs）,胰岛素敏感指数（ISI）,肝脏指数（LI）,C肽;苏木素-伊红（HE）染色观察肝脏病理变化;采用实时荧光定量PCR（Real-time PCR）检测肝组织miRNA-29a,FOXO3表达水平。结果：正常组大鼠饮食正常,皮毛整洁,精神状态良好,模型组大鼠精神萎靡、皮毛凌乱欠光泽,懒惰,麦冬提取物组及二甲双胍组大鼠皮毛欠光泽,有不同程度活动及饮食减少,精神状态优于模型组;麦冬提取物可明显降低胰岛素抵抗大鼠BW,LW,LI,C肽水平（P〈0.05）,明显降低TC,TG,FINs水平（P〈0.05）,显著升高ISI水平（P〈0.05）,改善大鼠胰岛素抵抗;麦冬提取物明显改善胰岛素抵抗模型大鼠脂肪变性,改善肝脏病理状态;麦冬提取物可明显下调miRNA-29a表达,上调FOXO3表达,且呈现一定剂量依赖性（P〈0.05）。结论：麦冬提取物可明显改善胰岛素抵抗,推测其机制为下调miRNA-29a表达,上调FOXO3表达。Objective： To investigate the effect of Ophiopogonis Radix extract on expressions of miRNA-29 a and FOXO3 in liver tissues of insulin resistant rats. Method： A total of 60 male SD rats were selected,including 10 for normal control. The remaining 50 rats were randomly divided into model control group,high,medium and low-dose Ophiopogonis Radix extract groups,metformin positive control group 5,with 10 in each group. High,medium and low-dose Ophiopogonis Radix extract groups were given 150,300,500 mg·kg^-1 Ophiopogonis Radix extract,the positive control group was given 200 mg·kg^-1 metformin suspension through intragastric administration for intervention,while the normal control group and the model control group were given the same volume of saline. The levels of body weight（ BW）,liver weight（ LW）,fasting blood glucose（ FBG）,total cholesterol（ TC）,triglyceride（ TG）,fasting insulin（ FINs）,insulin sensitivity index（ ISI）,liver index（ LI） and C-peptide were measured by hematoxylin-eosin（ HE） staining. The pathological changes in liver were observed,and the expressions of miRNA-29 a and FOXO3 in liver tissues were detected by Real-time PCR at the 4 th and 8 thweeks after administration. Result： The rats in the normal control group were given normal diets,with clean skin and in a good mental state. The rats in the model control group were lethargic and lazy,with messy and dim skin. The Ophiopogonis Radix extract and the metformin groups had dim skin,with a better mental state than the model control group. Ophiopogonis Radix extract significantly reduced the levels of BW,LW,C-peptide and LI in insulin resistance rats（ P〈0. 05）,significantly reduced the levels of TC,TG and FINs（ P〈0. 05）,significantly increased ISI levels（ P〈0. 05）,and improved insulin resistance in rats. Ophiopogonis Radix extract significantly relieved steatosis of rats in the insulin resistance model and pathological status of liver. Ophiopogonis Radix extract significantly down-regulated miR

关 键 词：麦冬提取物 胰岛素抵抗 miRNA-29a 转录因子FOXO3 

分 类 号：R285.5[医药卫生—中药学]