聚乙二醇化多西他赛白蛋白纳米粒用于非小细胞肺癌模型的实验研究  

Experimental research on docetaxel loaded PEG-albumin nanoparticles to the situ carcinoma model of non-small cell lung cancer

作　　者：金光明[1] 尹学哲[1] 金明姬 高钟镐 

机构地区：[1]延边大学附属医院,延吉133000 [2]中国医学科学院药物研究所,天然药物活性物质与功能国家重点实验室,药物传输技术及新型制剂北京市重点实验室,北京100050

出　　处：《中国新药杂志》2017年第23期2871-2876,共6页Chinese Journal of New Drugs

摘　　要：目的:制备聚乙二醇化多西他赛白蛋白纳米粒(PEG-albumin nanoparticles,PEG-DANPs),比较市售多西他赛(艾素~?,Aisu~?)、多西他赛白蛋白纳米粒(DANPs)和PEG-DANPs三者对裸鼠非小细胞肺癌模型的作用。方法:分别建立BALB/c裸鼠皮下移植瘤及原位癌模型,待皮下组肿瘤体积到达120 mm3时,两组模型分别通过尾静脉注射葡萄糖注射液,Aisu~?,DANPs和PEG-DANPs进行治疗,每7 d给药1次,共给药4次。在最后1次给药2 d后,将皮下移植瘤组和原位癌组所有裸鼠全部处死,取瘤块、肺脏、心脏、肝脏、脾送检HE(hematoxylin-eosin staining)染色切片。另选取裸鼠32只,建立皮下移植瘤模型,分别注射上述相同药物,持续治疗直至裸鼠自然死亡,最终统计并绘制裸鼠生存曲线。结果:PEG-DANPs能够有效地抑制裸鼠皮下移植瘤及原位癌的增殖,它能够有效地控制原位癌的转移并且延长裸鼠的生存时间。结论:PEGDANPs是一种抗肿瘤细胞效果明显、不良反应小的药物,具有广阔的应用前景。Objective：To prepare DANPs and PEG-DANPs and compare the effect of docetaxel commercial products（Aisu~？）,docetaxel-albumin nanoparticles（DANPs） and docetaxel-loaded PEG-albumin nanoparticles（PEG-DANPs） against the situ carcinoma model of non-small cell lung cancer（NSCLC） in mice.Methods：We established the transplantation tumor model and the situ carcinoma model of NSCLC.When the tumor volume in transplantation tumor group reached about 120 mm^3,the mice were treated 4 times at 7-day intervals with 5%glucose injection,Aisu~？,DANPs or PEG-DANPs,respectively.48 hours after the last treatment,the mice were sacrificed.The tumors with lung and the other major organs（including heart,liver,spleen,kidney） were removed and subjected to paraffin embedding for HE staining.Select another 32 of nude mice to establish the transplantation tumor model and treated them with the same drugs until to natural death,observe the survival rate and draw a rate curve.Results：The PEG-DANPs can effectively inhibit the growth of NSCLC transplanted tumor and situ carcinoma,it can suppress metastases more effectively and prolong the survival of the mice.Conclusion：PEG-DANPs has less side effect and its anti-tumor effect is more apparent,which has an extensive application psrospect.

关 键 词：非小细胞肺癌 A549细胞 多西他赛 白蛋白纳米粒 聚乙二醇 

分 类 号：R944[医药卫生—药剂学]