超氧化物歧化酶在甲状腺相关疾病外周血中的水平及意义  

Activity of serum superoxidein in patient with thyroid associate diseases

作　　者：徐艳秋[1] 刘斌[2] 常媛 宋晓斐[1] 赵锐[4] 栾芳[1] 

机构地区：[1]山东大学附属省立医院临床医学检验部,山东济南250021 [2]山东大学附属省立医院医学工程部,山东济南250021 [3]山东大学附属省立医院麻醉科,山东济南250021 [4]山东大学齐鲁医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》2017年第9期680-683,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金青年基金项目(81101484);山东省优秀中青年科学家奖励基金(BS2011SW035)

摘　　要：目的:研究超氧化物歧化酶(SOD)在甲状腺相关疾病外周血中水平,分析与甲状腺疾病的关系。方法:甲状腺癌患者84例,结节性甲状腺肿患者21例,甲状腺功能亢进患者75例,甲状腺功能低下患者56例和正常人对照组63例,酶法检测外周血清中SOD活性,电化学发光法检测游离甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平,分析各组SOD活性差异及SOD活性与甲状腺激素相关性。结果:SOD活性在甲状腺癌组(164.536±18.095)U/mL,明显低于甲状腺功能亢进症组的(173.376±15.942)U/mL、甲状腺功能低下症组(174.827±19.895)U/mL和正常对照组的(179.529±11.625)U/mL(P<0.05),SOD活性在结节性甲状腺肿组(157.667±15.189)U/mL,明显低于甲状腺功能亢进症组的(173.376±15.942)U/mL、甲状腺功能低下症组(174.827±19.895)U/mL和正常对照组的(179.529±11.625)U/mL(P<0.05)。甲状腺癌组FT3、FT4水平与SOD活性成呈正相关(r=0.346、0.328,P<0.05),正常对照组FT3水平与SOD活性成呈负相关(r=-0.552,P<0.05)。结论:甲状腺发生结节和肿瘤后SOD活性降低,观察SOD活性变化可了解甲状腺组织损伤的情况并作为甲状腺肿瘤发生辅助指标。Objective： To study the activity of superoxide dismutase （dismutase superoxide, SOD） in peripheral blood of patients with thyroid associated diseases. Methods： 84 cases of pa- tients with thyroid cancer, 21 cases of patients with nodular goiter, 75 cases of patients with hyper- thyroidism, 56 cases of patients with hypothyroidism and 63 cases of normal control were collected, serum enzyme activity of SOD was detected by enzyme method, FT3, FT4 and TSH levels were de- tected by electrochemiluminescence assay. Student-t, Mann-whitney-U or Kruskal-Wallis was ap- plied to analyse the different of SOD activity in different groups. Pearson correlation analysis were applied to analyze the correlation of SOD activity with thyroid hormone levels. Results： SOD activity in thyroid cancer group （164.536 ± 18.095） U/ml was significantly lower than that in hyperthyroidism group （173.376 ± 15.942） U/ml, hypothyroidism group （174.827 ± 19.895） U/ml and normal control group（179.529 ±11.625） U/mI（P〈0.05）, SOD activity in nodular goiter group（157.667 ± 15.189） U/ml was significantly lower than that in hyperthyroidism group （173.376 ± 15.942） U/ml, hypothyroidism group （174.827 ± 19.895） U/ml and normal controls （179.529 ±11.625） U/mi （P〈0.05）. There were positive correlation of FT3, FT4 levels respectively with SOD activity in thyroid cancer group（r=0.346 P〈0.05, r=0.3278,P〈0.05）, and there was positive correlation of FT3 level with SOD activity in normal control group （r=-0.5522.P〈0.05）. Conclusions： SOD activity decreased in nodular goiter and thy- roid cancer, so it could be used to observe the damage of thyroid tissue, Furtherly, it might be used as an auxiliary index in the diagnosis of thyroid tumors.

关 键 词：超氧化物歧化酶 甲状腺肿瘤 结节性甲状腺肿 甲状腺功能亢进 甲状腺功能低下 甲状腺激素 

分 类 号：R653[医药卫生—外科学]