利用诱导多能干细胞构建类肾体的方法  

Kidney organoids generated from induced pluripotent stem cells

作　　者：肖枫林 李清刚[1] 傅博[1] 吴玲玲[1] 马倩 沈婉君 陈香美[1] 

机构地区：[1]解放军总医院肾脏病科,北京100853

出　　处：《中国医药》2017年第12期1861-1864,共4页China Medicine

基　　金：国家自然科学基金（81330019）

摘　　要：目的利用诱导多能干细胞（iPSCs）向肾系细胞定向诱导分化，并获得类。肾体。方法利用肝糖原合成激酶3B受体的选择性抑制剂（CHIR99021）及成纤维细胞生长因子9对iPSCs进行定向诱导分化，控制后肾间充质与输尿管上皮的形成比例，从而构建类肾体。对类肾体细胞进行免疫荧光染色，利用共聚焦激光扫描显微镜观察并拍摄照片。利用扫描电镜观察类肾体的内部结构，并拍摄照片。结果利用iPSCs分化成功构建出类肾体。通过结构鉴定，证实类肾体中包括肾单位的各个部分。结论利用iPSCs进行定向诱导分化可以获得包含远端小管、近端小管、肾小球及血管的类。肾体。Objective To obtain kidney organoids from induced pluripotent stem cells （iPSCs） differentiation. Methods Glycogen synthase kinase-313 receptor selective inhibitor CHIR99021 and fibroblast growth factor-9 were used to induce the differentiation of iPSCs and control the ratio between metanephrie mesenchyme and ureteral epithelium to construct kidney organoids. Kidney specific markers in kidney organoids were detected through immunofluorescence staining by confocal laser scanning microscope. Internal microstructures of kidney organoids were observed by scanning electron microscope. Results Kidney organoids containing various parts of nephron were successfully generated from the differentiation of iPSCs. Conclusion Kidney organoids containing distal and proximal tubules, glomerulus and vasculatures can be obtained by the differentiation of iPSCs.

关 键 词：诱导多能干细胞 细胞分化 类肾体 

分 类 号：R34[医药卫生—基础医学]