黄连多糖中单糖组成的HPLC-MS^n法快速识别  

Monosaccharide Composition Analysis of Polysaccharides from Rhizoma Coptidis Based on HPLC-MS^n

作　　者：张亚丽[1] 高简 苗祥贞 张潇 刘永刚[1] 谭鹏[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《世界中医药》2017年第11期2775-2778,共4页World Chinese Medicine

基　　金：青年科学基金项目(81102807)

摘　　要：目的:探讨采用HPLC-MS^n法快速识别黄连多糖中的单糖组成。方法:黄连药材经过提取、分离、除蛋白、除色素,最终得到黄连多糖,用2 mol/L的三氟乙酸水解黄连多糖,以1-苯基-3-甲基-5-吡唑啉酮(PMP)作单糖衍生化试剂,利用HPLC-MS^n识别黄连中单糖的组成,并解析其衍生物的裂解规律。结果:通过分析,得到的黄连多糖中含有甘露糖、核糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖/木糖、岩藻糖,其中核糖、岩藻糖为首次在黄连多糖中报道的单糖。结论:HPLC-MS^n法对中药多糖组分中的单糖成分进行分析具有重要的指导意义。Objective： To explore a rapid identification of monosaccharide composition of polysaccharide from Rhizoma Coptidis through HPLC-MS^n. Methods： After being extracted,separated,removed of protein and pigment,Rhizoma Coptidis polysaccharide was obtained from Rhizoma Coptidis. Polysaccharides of Rhizoma Coptidis was hydrolyzed with the TFA of 2 mol. L^（-1）. 1-phenyl-3-methyl-5-pyrazolone（ PMP） was used as derivatization reagent. And high performance liquid chromatography-mass spectrometry（ HPLC-MS^n） was used to identify the composition of monosaccharide,and the resolve fragmentation regularity of its derivatives was analyzed. Results： Through analysis,the monosaccharides of polysaccharides from Rhizoma Coptidis were mannose,ribose,rhamnose,galacturonic acid,glucose,galactose,arabinose/xylose,fucose,among which ribose and fucose were the first reported monosaccharides that obtained from polysaccharide of Rhizoma Coptidis. Conclusion： HPLC-MS^nhas a guiding significance in analyzing the monosaccharide composition of polysaccharides from Chinese materia medica.

关 键 词：黄连多糖 PMP 单糖 HPLC-MSn 

分 类 号：R284[医药卫生—中药学]