慢性低O_2高CO_2对小鼠海马细胞凋亡、caspase-3和p38 MAPK活性的影响  

Effect of the chronic hypoxic-hypercapnia in apoptosis and activity of caspase-3 and p38 MAPK of hippocampus in mouse

作　　者：任惠明[1] 王小同[2] 袁海[3] 康翰林 

机构地区：[1]宁波市第二医院康复医学科,浙江宁波315010 [2]温州医科大学附属第二医院脑科康复中心,浙江温州325027 [3]合肥市第二人民医院康复医学科,安徽合肥230011 [4]赣南医学院第一附属医院神经内科,江西赣州341000

出　　处：《温州医科大学学报》2017年第12期895-897,共3页Journal of Wenzhou Medical University

摘　　要：目的:研究慢性低O_2高CO_2对小鼠海马细胞凋亡、caspase-3以及p38 MAPK活性的影响。方法:雄性C57BL/6小鼠24只,随机分成2组:4HH组(低O_2高CO_2组12只)和NC组(正常对照组12只)。4HH组饲养于氧舱中,舱内O_2浓度为9%~11%,CO_2浓度为5%~6%,每天8 h,每周6 d,共4周,其余时间与NC组一样,生活于正常环境。原位末端标记(TUNEL)法检测海马细胞凋亡指数(AI),caspase-3活性检测试剂盒测定海马caspase-3活性,Western blot检测海马p-p38 MAPK蛋白的表达。结果:4HH组小鼠海马细胞凋亡指数(AI)、caspase-3活性及p-p38 MAPK蛋白含量显著高于NC组(P<0.01)。结论:慢性低O_2高CO_2促进小鼠海马细胞凋亡,促进caspase-3以及p38 MAPK活化。Objective： To investigate the effect of the chronic hypoxic-hypercapnia in apoptosis and activity of p38 MAPK and caspase-3 of hippocampus in mouse. Methods： Twenty-four male mice were randomly divided into two groups： the hypoxic-hypercapnia 4 weeks （4HH） group and normal control （NC） group. The 4HH groups in the special cantainer were exposed to an atmosphere containing 9% - 11% 02 and 5% - 6% CO2 8 hours a day, 6 days a week for 4 weeks. The two groups lived in the same room in the rest time. Apoptosis index （AI） in hip- pocampus was counted by TUNEL staining. Caspase-3 activity was measured by a caspase-3 activity assay kit. p-p38 MAPK protein in hippocampus was measured by Western blot. Results： Compared with NC group the AI, caspase-3 activity, and the p-p38 MAPK protein expression in hippocampus were significantly increased in 4HH group （P〈0.01）. Conclusion： The chronic hypoxic-hypercapnia promotes apoptosis and the activation of cas- pase-3 and p38 MAPK of hippocampus in mouse.

关 键 词：低氧 高碳酸血症 P38丝裂原活化蛋白激酶类 CASPASE-3 凋亡 小鼠 海马 

分 类 号：R338.2[医药卫生—人体生理学]