具有微生物颗粒预富集功能的微过滤系统设计  

Design of a Microfilter System for Microbiological Particle Preconcentration

作　　者：杨潞霞 张斌珍[2,3] 段俊萍[2,3] Yang Luxia;Zhang Binzhen;Duan Junping(School of Information, Business College of Shancci University, Taiyuan 030031, China;Key Laboratory of Instrumentation Science ~ Dynamic Measurement of Ministry of Education;Science and Technology on Electronic Test and Measurement Laboratory, North University of China, Taiyuan 030051, China)

机构地区：[1]山西大学商务学院信息学院,太原030031 [2]中北大学仪器科学与动态测试教育部重点实验室,太原030051 [3]中北大学电子测试技术重点实验室,太原030051

出　　处：《微纳电子技术》2018年第1期26-31,共6页Micronanoelectronic Technology

基　　金：国家自然科学基金面上项目(61176115);国家自然科学基金资助项目(51475438);山西省青年科技研究基金面上青年基金资助项目(201701D221103);山西大学商务学院科研基金计划项目(2017009)

摘　　要：为了提高微流式细胞仪对低浓度细胞样本液的检测效率和准确率,提出一种以电磁驱动的微过滤系统设计方案。该微过滤系统包括具有过滤结构的微流控芯片（微过滤芯片）和电磁驱动机构,借助电磁控制来实现微过滤芯片对微生物颗粒的富集功能。使用微电子机械系统（MEMS）工艺设计与制作了聚二甲基硅氧烷（PDMS）薄膜微过滤芯片,通过对微过滤芯片的受力分析,自制了一套电磁驱动装置。使用溶有直径为30μm荧光微球的去离子水溶液（荧光微球与去离子水的体积比1∶40）对微过滤系统样机进行功能测试,通过控制电流大小、通电时间对给定溶液中的荧光微球实现了预富集效果。测试结果表明该微过滤系统设计合理,具有制作简单,成本低、易于集成等优点,能够在医学检测中广泛应用。In order to improve the testing efficiency and accuracy of the cell sample solution with low concentration using the microfluidic cytometer,a design scheme of the electromagnetic drive microfilter system was presented. The microfilter system consisted of the microfluidic chip with the filtering structure（microfilter chip） and electromagnetic drive device,and the microbial particle enrichment function of the microfilter chip was realized with the help of the electromagnetic control. The micro-electromechanical system（ MEMS） technology was used to design and fabricate the polydimethylsiloxane（ PDMS） membrane microfilter chip. Through the force analysis of the microfilter chip,the electromagnetic drive device was fabricatied. The microfilter system prototype was tested using the deionized water solution with 30 μm diameter fluorescent microsphere（ the volume ratio of the fluorescent microsphere and deionized water was 1∶ 40）,the preconcentration effect of the fluorescent microsphere in the solution was achieved by controlling the current and conduction time. The test results show that the design of the microfilter system is reasonable,has advantages of simple fabrication process,low production cost and ease of integration,and can be widely applied in the medical testing.

关 键 词：预富集 过滤 微流控芯片 聚二甲基硅氧烷(PDMS) 电磁驱动 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置] TH703[自动化与计算机技术—控制科学与工程]