黄芩拆分组分寒热药性归属  

Cold and Warm Property of Scutellaria Baicalensis Split Componet

作　　者：张亚男[1] 陈平平[1] 王洪玉[1] 高鑫[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2017年第12期32-36,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531804)

摘　　要：目的 :通过检测热证模型大鼠体内物质代谢、能量代谢、内分泌系统及植物神经系统相关酶的含量,考察黄芩拆分组分对其表达的影响,以阐述其寒热属性。方法 :将SD雄性大鼠随机分为空白对照组、模型组、全成分组、苷元组、苷类组、多糖组。连续灌胃给药15 d,留取肝脏、心脏、肌肉等脏器,检测物质代谢相关酶葡萄糖激酶(GCK)、果糖磷酸激酶(PFK)、丙酮酸激酶(PK)、乙酰辅酶A(AC-COA)、丙酮酸脱氢酶(PDH)、柠檬酸合酶(CS)、异柠檬酸脱氢酶(ICD)、糖原合成酶激酶-3(GSK-3)、糖原磷酸化酶(PYGL)、脂肪甘油三酯脂酶(ATGL)及能量代谢相关酶腺甘酸激酶(ADK)、ATP合成酶(ATPs)、细胞色素C还原酶(CCR)、细胞色素C氧化酶(COX)、NaK-ATP酶(肌肉)、Na-K-ATP酶(肝脏)的表达。检测内分泌系统相关的三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)以及植物神经系统相关的5-羟色胺(5-HT)、乙酰胆碱(Ach E)、去甲肾上腺素(NE)的表达。结果 :模型组GCK、PFK、PK、PDH、CS、ICD、GSK-3、ATPs、CCR、COX、ADK、NA-K-ATP(肝脏)、NA-K-ATP(肌肉)、T3、T4、Ach E、NE的表达显著上升(P<0.05);AC-COA、PYGL、ATGL、5-HT的表达显著下降(P<0.05)。全成分组GCK、PFK、PK、PDH、CS、GSK-3、COX、ADK、T3、T4、Ach E、NE的表达显著下降(P<0.05);AC-COA、5-HT的表达显著升高(P<0.05)。苷元组GCK、PFK、PK、PDH、CS、GSK-3、ADK、NA-K-ATP(肌肉)、T3、Ach E、NE的表达显著下降(P<0.05);5-HT的表达显著升高(P<0.05)。苷类组GCK、PFK、PDH、CS、ICD、GSK-3、ADK、ATPs、CCR、COX、NA-K-ATP(肝脏)、NA-K-ATP(肌肉)、T4、Ach E的表达显著下降(P<0.05);5-HT的表达显著升高(P<0.05)。结论 :黄芩全成分能够抑制物质代谢、能量代谢、内分泌系统及植物神经系统,再一次证实了黄芩其寒凉属性,与本课题首席科学家匡海学提出的新假说相契合;总体来看,苷元和苷类与寒凉中药具有相似的作用趋势Objective ：To explore the cold and warm property of Scutellaria baicalensis split componet by detecting related enzyme expression in substance metabolism,energy metabolism,endocrine system and autonomic nervous system in heat syndrome model rats.Methods ：The 60 male SD rats were randomly divided into blank control group,model group,total composition group,aglycone group,glycosides group,polysaccharide group.All groups ig for 15 days.Taking the liver,heart,muscle and detecting the related enzyme,glucokinase（GCK）,phosphofructokinase（PFK）,pyruvate kinase（PK）,acetyl coenzyme A（AC-COA）,pyruvate dehydrogenase（PDH）,citrate synthase（CS）,isocitrate dehydrogenase（ICD）,glycogen synthase kinase-3（GSK-3）,glycogen phosphorylase（PYGL）and adipose triglyceride lipase（ATGL）and energy metabolism related enzyme adenosine kinase（ADK）,ATP synthetase（ATPs）,cytochrome C reductase（CCR）and cytochrome C oxidase（COX）,Na-K-ATP enzyme（muscle）,Na-K-ATP enzyme（liver）expression.Detection of three triiodothyronine related endocrine system（T3）,four triiodothyronine（T4）and serotonin 5-autonomic nervous system related（5-HT）and acetylcholine（Ach E）,norepinephrine（NE）expression.Results ：The model group,GCK,PFK,PK,PDH,CS,ICD,GSK-3,ATPs,CCR,COX,ADK,NA-K-ATP（liver）,NA-K-ATP（muscle）,T3,T4,Ach E,NE were significantly increased（P〈0.05）;AC-COA,PYGL,ATGL,5-HT were significantly decreased（P〈0.05）.The total composition group GCK,PFK,PK,PDH,CS,GSK-3,COX,ADK,T3,T4,Ach E,NE were significantly decreased（P〈0.05）;AC-COA,5-HT were significantly increased（P〈0.05）.The aglycone group,GCK,PFK,PK,PDH,CS,GSK-3,ADK,NA-K-ATP（muscle）,T3,Ach E,NE were significantly decreased（P〈0.05）,5-HT was significantly increased（P〈0.05）.The glycosides group,GCK,PFK,PDH,CS,ICD,GSK-3,ADK,ATPs,CCR,COX,NA-KATP（liver）,NA-K-ATP（muscle）,T4,Ach E was significantly decreased（P〈0.05）;5-HT were significantly increased（P〈0.05）.Conclusion ：Total c

关 键 词：黄芩拆分组分 寒热药性 物质代谢 能量代谢 内分泌系统 植物神经系统 

分 类 号：R285.1[医药卫生—中药学]