回医烙灸疗法对大鼠腰椎间盘退变模型软骨细胞凋亡的影响  

Effect of Moxibustion in Huiyi on Articular Chondrocyte Apoptosis of Lumbar Intervertebral Disc Degeneration in Rats

作　　者：许建峰[1,2] 白帅[1] 林瑞珠 武永利[1,2] 牛子瞻[1] 王英絮[1] 

机构地区：[1]宁夏医科大学总医院,宁夏银川750004 [2]宁夏医科大学回医药现代化教育部重点实验室,宁夏银川750004

出　　处：《辽宁中医药大学学报》2017年第12期63-65,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81360567);宁夏医科大学优势学科群建设科研项目(XY201622)

摘　　要：目的 :观察回医烙灸疗法对大鼠腰椎间盘退变模型软骨细胞凋亡及相关凋亡因子的影响。方法 :将SD大鼠40只,随机分为正常对照组、假手术组、模型对照组和回医烙灸组,每组各10只。采用针刺间盘方法制备大鼠腰椎间盘退变模型。回医烙灸组在造模4周后,在腰椎局部行回医烙灸疗法,每周2次,每次20 min,干预6周。正常对照组、假手术组和模型组不予干预。4组动物干预8周后处死取材,软骨细胞凋亡用TUNEL法检测,Bcl-2、Bax、Caspase-3蛋白的表达采用免疫组化方法进行检测。结果 :回医烙灸组凋亡指数明显低于模型对照组(P<0.05);回医烙灸组和正常对照组、假手术组比较,差异无明显统计学意义(P>0.05)。回医烙灸组间盘软骨组织Bax和Caspase-3的阳性细胞平均数低于模型组(P<0.05),软骨组织Bcl-2的阳性细胞平均数高于模型对照组(P<0.05)。结论 :回医烙灸疗法可以减少大鼠腰椎间盘退变模型软骨细胞的凋亡,并可以调节软骨细胞凋亡相关因子的表达,从而延缓腰椎间盘的退变。Objective ：To observe the effect of moxibustion in Huiyi on articular chondrocyte apoptosis of lumbar intervertebral disc degeneration in rats and apoptosis factor.Methods ：A total of 40 adult SD rats were randomly divided into normal group,sham operation group,animal model group and moxibustion group.Each group was 10 rats.The experimental lumbar intervertebral disc degeneration was constructed by needling.The animals of moxibustion group were treated with moxibustion 8 weeks,then all the rats were executed and draw material.The changes of articular chondrocyte apoptosis of lumbar intervertebral disc were observed by TUNEL.The Bcl-2,Bax and Caspase-3 were detected by immunohistochemical.Results ：The chondrocyte apoptosis change in normal group,sham operation group and moxibustion group were better than one in the animal model group（P〈0.05）.The expression of Bcl-2 in normal group,sham operation group and moxibustion group were higher than one in the animal model group（P〈0.05）.The expression of Bax in normal group,sham operation group and moxibustion group were lower than one in the animal model group（P〈0.05）.Conclusion ：Moxibustion in Huiyi can control the expression of chondrocyte apoptosis in the experimental lumbar intervertebral disc degeneration model,and can regulate apoptosis factor.It maybe delays and inhibits the degeneration of chondrocyte.

关 键 词：回医烙灸疗法 腰椎间盘退变 软骨细胞 凋亡 

分 类 号：R681.53[医药卫生—骨科学]