中度海拔地区汉族人群男性不育患者精子中Chk1基因的表达变化及机制  

Expression of sperm Chk1 gene in infertility men in Han nationality living at middle altitude area

作　　者：张树娜[1] 李燕子[1] 杨惠林[1] 黄文军[1] 腾小娜 ZHANG Shu-na;LI Yan-zi;YANG Hui-lin;HUANG Wen-jun;TENG Xiao-na(Qinghai red cross hospital, Xinin)

机构地区：[1]青海红十字医院,西宁810000

出　　处：《中国高原医学与生物学杂志》2017年第4期280-284,共5页Journal of Chinese High Altitude Medicine & Biology

摘　　要：目的探讨中度海拔地区高原低氧环境影响男性精液质量是否与Chk1表达变化有关。方法收集正常精子组、少精子组、弱精子组和少弱精子组的标本各20份,采用qRT-PCR和Western blot方法,从mRNA和蛋白水平检测各组精子中Chk1的表达,并对相关结果行统计学分析。结果 1)在各组精子中均可检测到Chk1蛋白的表达;2)少精子组、弱精子组和少弱精子组精子中Chk1mRNA及蛋白表达量显著低于正常精子组(P<0.05),且在少弱精子组表达最低。结论Chk1在中度海拔地区汉族人群男性不育患者精子中表达下调,可能与高原低氧环境影响精液质量有关。Objective To investigate whether the semen checkpoint kinase 1 （ Chkl ） expression affected by the hypoxia environment in the middle altitude area.Methods 80 semen samples were collectedand divided them into 4 groups ： normal （ 20 ）, oligozoospermia （ 20）, asthenospermia （ 20 ） and oligoasthenozoospermia （ 20 ）. The expression of Chkl mRNA and protein in sperm were detected by qRT-PCR and Western blot.Results 1） Sperm Chkl gene were positively expressed in all of the groups ;2）The expression of Chkl mRNA and protein in oligozoospermia,as- thenospermia and oligoasthenozoospermia were significantly lower than that in the normal（P〈0.05） , and the lowest level in the oligoasthenozoospermia.Conclusion The expression of sperm Chkl gene in infertility men in Han nation- ality living in middle altitude is considered down-regulated, suggesting that it may be the reason for effecting on se- men quality with the altitude hypoxia environment.

关 键 词：细胞周期检测点激酶1 中度海拔地区 不育精子 

分 类 号：R698.2[医药卫生—泌尿科学]