鲜土贝母二氯甲烷提取物对人乳腺癌MDA-MB-231-GFP裸鼠肿瘤生长及肺转移的抑制作用  

Inhibitory Effect of Fresh Tubeimu(Rhizoma Bolbostemmae) Dichloromethane Extract on Tumor Growth and Lung Metastasis of Human Breast Cancer MDA-MB-231-GFP Nude Mice

作　　者：鲍伟倩 王婧筱 安超[1] 胡凯文[1] 杨萌 Robert M.Hoffman 齐慧 曹阳[1] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]安泰康生物技术(北京)有限公司,北京102200 [3]美国加州大学外科系,美国圣地亚哥92103

出　　处：《中医药导报》2017年第23期18-21,34,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(81303129);高等学校博士学科点专项科研基金(20120013120013);北京中医药大学中西医结合肿瘤治疗协同创新中心(2013-XTCX-05)

摘　　要：目的:研究土贝母鲜品二氯甲烷提取物(DEFT)对绿色荧光蛋白标记的人三阴性乳腺癌MDA-MB-231-GFP裸鼠模型中移植瘤生长和肺转移的作用。方法:应用外科原位移植方法,建立人乳腺癌裸鼠移植模型,随机分成3组,阴性对照组(0.9%Na Cl)、低剂量组(DEFT 50 mg/kg)和高剂量组(DEFT 75 mg/kg),每组5只,灌胃给药,1次/d,连续58 d。每周2次荧光成像系统釆集肿瘤图像,系统自带软件计算肿瘤面积;给药结束时,称取肿瘤质量,荧光成像系统观察肺转移情况。结果:给药前后3组裸鼠体质量变化差异均无统计学意义(P>0.05)。给药第14天,3组肿瘤面积比较,差异有统计学意义(P<0.05)。实验结束时,土贝母低剂量组和高剂量组肺转移的发生均低于阴性对照组(P<0.05)。土贝母低剂量组和高剂量的肿瘤质量低于阴性对照组,但差异无统计学意义(P>0.05)。结论:鲜土贝母二氯甲烷提取物对人三阴性乳腺癌MDA-MB-231-GFP裸鼠模型中肿瘤生长及肺转移的发生有一定抑制作用。Objective： To study the effect on the growth of transplanted tumor and lung metastasis in a green fluorescent protein marking human triple-negative breast cancer MDA-MB-231-GFP nude mice model when treated with the dichloromethane extract of fresh Tubeimu （Rhizoma Bolbostemmae） （DEFT）. Methods： The nude mice model was established by surgical orthotopic transplantation method. The mice were randomly divided into 3 groups （5 mice in each group）： negative control group （0.9% sodium chloride）, low dose group （DEFT, 50 mg/kg） an（/ high dose group （DEFT, 75 mg/kg）. Each group was treated with oral administration once a day tor 58 days. The tumor image was collected by the fluorescence imaging system twice each week, and the soft- ware was used to calculate the tumor area. At the end of the treatment, the tumor weight was recorded and the lung metastasis was observed by the fluorescence imaging system. Results： Weight changes between the control group and the treatment groups were not significantly different before and after. The tumor area between three groups began to grow significantly differently after 14 days （P〈0.05）. Lung metastasis rates decreased significant- ly at the end of the experiment （P〈0.05）. The treatment groups showed less tumor weight than the control group,but there was no significarit difference （P〉0.05）. Conclusion： The dichloromethane extract of fresh Tubeimu （Rhiz6ma Bolbosfemmae） had Some inhibitory effect on the growth of malignant tumors and the occurrence of lung metastasis in human triple-iaegative breast cancer MDA-MB-231-GFP nude mice model.

关 键 词：土贝母提取物 中药提取物 乳腺癌 人乳腺癌MDA-MB-231-GFP祼鼠模型 肺转移 

分 类 号：R273[医药卫生—中西医结合]