胶体金快速检测技术在布鲁杆菌病中的应用  

Clinical application of colloidal gold rapid detection techniquein the diagnosis of human brucellosis

作　　者：周玉珍[1] 毕惠梅[1] 许慧娇 周坤 王立庄 Zhou Yuzhen;Bi Huimei;Xu Huijiao;Zhou Kun;Wang Lizhuang(Clinical Laboratory of Heilongjiang Agricultural Reclamation Bureau General Hospital, Harbin 150088, China)

机构地区：[1]黑龙江省农垦总局总医院检验科,哈尔滨150088

出　　处：《国际免疫学杂志》2017年第6期635-637,共3页International Journal of Immunology

摘　　要：目的探讨胶体金快速检测技术在布鲁杆菌病诊断中的临床应用意义。方法选取黑龙江省农垦总局医院就诊的布鲁杆菌病患者和健康体检者为研究对象，分别用胶体金快速检测布病抗体、虎红、试管凝集试验和血培养进行检测。结果胶体金快速检测布病抗体与虎红、试管凝集试验的Kappa值为0．796、0．769，Kappa值〉0．75有较好的一致性，胶体金与血培养检测结果的比较Kappa值为0．742，Kappa值在0．4～0．75之间，两种检测方法的一致性一般。胶体金快速检测布病抗体的诊断符合率和灵敏度都高于虎红。结论胶体金快速检测布病抗体实用于布鲁杆菌感染人群的初筛普查。Objective To explore the application value of colloidalgold rapid detection techniquefor the diagnosis of Brucellosis. Methods Patients with Brucellosis treated in our hospitalwere enrolled in this study. Healthy people having annualphysical examination were recruited as control. Antibody of Brucella was detected by colloidal gold rapid detection. Other detection such as Tiger Red, serum agglutination test（SAT） and blood culture were also performed. Results The Kappa value for colloidal gold rapid detection, tiger red, and SAT was 0. 796 and 0. 796, respectively. Good consistency in Kappa value （ 〉 0.75 ） was obtained. The results of colloi- dal gold were compared with that of the blood culture. The Kappa value was 0. 742, which was between 0. 4 to 0. 75,indicating the less consistency of the two methods. Diagnostic coincidence and sensitivity rate through de- tecting brucella antibodies bycolloidal gold rapid detection were higher than that by using red tiger. Conclusions Detection of brucella antibodies by colloidal gold rapid detection technique might be used for the preliminary population survey of brucella infection.

关 键 词：布鲁杆菌病 胶体金快速检测布病抗体 虎红 试管凝集试验 

分 类 号：R446.6[医药卫生—诊断学] R516.7[医药卫生—临床医学]