APPswe/PS1dE9转基因小鼠小脑内突触素及BDNF/Trk-B蛋白表达的变化  

Expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice

作　　者：董雪帆 王婷[1] 李甜[1] 焦娟娟[1] 曲雪松[1] 祁金顺[1] 杨威[1] 

机构地区：[1]山西医科大学生理学系细胞生理学省部共建教育部重点实验室,太原030001

出　　处：《中国应用生理学杂志》2017年第6期488-492,I0015,I0016,共7页Chinese Journal of Applied Physiology

基　　金：山西省回国留学人员科研资助项目(2016060)

摘　　要：目的:观察APP/PS1转基因小鼠小脑突触素及BDNF/Trk-B蛋白表达变化。方法:选用9月龄APP/PS1雄鼠(n1)和同窝对照野生型WT雄鼠(n2)。采用Western blot(n1=6;n2=6)、免疫组化(n1=4;n2=4)两种方式定量、定位测定小脑组织功能活性依赖蛋白突触素、脑源性神经营养因子(BDNF)和其高亲和力受体(Trk-B)的蛋白表达。用透射电镜观察小脑皮质突触超微结构变化(n1=2;n2=2)。结果:与WT组相比,APP/PS1组小脑皮质内突触素、BDNF/Trk-B表达明显减少;突触间隙增宽,突触后致密区变薄,密度降低。结论:APP/PS1小鼠小脑皮质中突触素、BDNF/Trk-B蛋白含量均明显降低,突触超微结构也发生明显改变,提示AD小脑突触数量及形态变化可能与BDNF合成及释放减少有关。Objective： To observe the expressions of synaptophysin and BDNF/Trk-B in eerebelhnn of APPswe/PSldE9 transgenic mice. Methods： The healthy 9-month old APP/PSI male mice （nl） and the same wild type male mice（n2） were divided into two groups, APP/PSl group and wild-type（WT） group. The expressions of synaptophysin and brain-derived neurotrophic factor/tyrosine kinase B （BDNF/Trk-B） in cerebellum were determined by Westena blot （nl = 6; n2 = 6） and immunohistochemical（ nl = 4; n2 = 4）. The possible synaptic changes in APP/PSI group were observed by using electron microscopy. Results： Compared with WT group, the expressions of synaptophsin and BDNF/ Trk-B in cerebellum were decreased in APP/PS1 group. Increased width of the synaptic cleft and decreased thickness of postsynaptic density were also observed, Conclusion： In APP/PSI group, expressions of synaptophsin and BDNF/Trk-B in cerebellum were decreased; changes in ultrastructure of synapses seemed to be widespread alterations, These findings suggest a possible association between expression of BDNF/TrkB and synaptie plasticity in AD cerebellum,

关 键 词：APP/PS1转基因小鼠 小脑 突触素 BDNF/Trk-B 突触 

分 类 号：Q0[生物学]