CLC-3 siRNA对人肺动脉平滑肌细胞增殖及细胞周期的影响  

CLC-3 siRNA effects on Human Pulmonary Artery Smooth Muscle Cells Proliferation and cell cycles

作　　者：师庆红[1] 秦迪[2] 赵长福[3] 张欣[2] 杨照微[1] 黄丽红[2] 何成彦[1] 

机构地区：[1]吉林大学中日联谊医院检验科,吉林长春130033 [2]吉林大学中日联谊医院老年病科,吉林长春130033 [3]吉林大学中日联谊医院骨科,吉林长春130033

出　　处：《中国实验诊断学》2017年第12期2171-2173,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金项目(81572082);吉林省科技厅资助(2015010153JC;20140311092YY)

摘　　要：目的探讨CLC-3siRNA对人肺动脉平滑肌细胞(PASMC)增殖及细胞周期的影响。方法应用脂质体3000将CLC-3siRNA转入PASMC。通过免疫印记法测定了CLC-3siRNA干扰后PASMC的CLC-3表达水平;应用台盼蓝染色计数法计数了细胞增殖;流式细胞仪测定了细胞周期。结果(1)CLC-3siRNA可降低PASMC的CLC-3表达;(2)CLC-3siRNA干扰可显著抑制PASMC增殖,与空白对照、阴性对照siRNA转染组相比P<0.05;(3)CLC-3siRNA干扰可显著减少PASMC的S期细胞、增加G1期细胞,与空白对照、阴性对照siRNA转染组相比P<0.05。结论 CLC-3siRNA干扰PASMC表达CLC-3,可抑制PASMC的增殖并影响其细胞周期进程。Objective To evaluate the effects of CLC-3 siRNA on the proliferation and cell cycles of PASMC.Meth-ods We transfect CLC-3 siRNA into PASMC by Lipofectamine 3000 reagent.The expression of CLC-3 protein in PASMC is detected by western blotting after CLC-3 siRNA transfection.The cell proliferation of PASMC is counting by trypan blue staining and the cell cycle of PASMC is analyzed by flow cytometry.Results （1）CLC-3 siRNA can re-duce the expression of CLC-3 protein in PASMC.（2）CLC-3 siRNA interference can significantly decrease the prolifera-tion of PASMC,compare to blank control,negative siRNA,P 〈0.05.（3）CLC-3 siRNA interference can significantly re-duce the S phase PASMCs and increase G1 phase PASMCs,compare to blank control,negative siRNA ,P 〈0.05.Con-clusion CLC-3 siRNA can interfere the CLC-3 expression in PASMC,and can decrease the proliferation of PASMC and affect the process of PASMC cell cycle.

关 键 词：肺动脉高压 肺动脉平滑肌细胞(PASMC) 氯离子通道CLC-3 SIRNA 

分 类 号：R544.1[医药卫生—心血管疾病]