CD4^＋T细胞通过分泌Th2型细胞因子介导RSV感染所诱发的气道炎症反应  

CD4^＋T cells mediate respiratory syncytial virus（RSV）-induced airway inflammation through secre-ting Th2 cytokines

作　　者：白松 崔玉琳 柴若楠[2] 范越 刘北星 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110122 [2]沈阳军区总医院呼吸内科与变态反应疾病诊治中心,110015 [3]锦州医科大学宣传部,121001

出　　处：《中华微生物学和免疫学杂志》2017年第10期747-752,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81671569）

摘　　要：目的 证实CD4^＋T细胞在呼吸道合胞病毒（respiratory syncytial virus,RSV）感染所诱发的气道炎症反应中的作用.方法 建立RSV急性感染的实验动物模型;采用HE染色观察肺病理炎症反应;流式细胞术分析脾组织中CD4^＋T细胞及分泌Th1/Th2型细胞因子IFN-γ、IL-4、IL-5、IL-13的CD4^＋T细胞数量;CD4^＋T细胞过继回输进一步证明其功能.结果 RSV感染后脾组织内CD4^＋T细胞总数,特别是分泌Th2型细胞因子的CD4^＋T细胞数量显著增加.CD4＋T细胞回输组较未回输组肺炎性细胞浸润明显,脾组织IL-4、IL-5、IL-13 mRNA表达水平明显增多.结论 证实CD4^＋T细胞通过分泌Th2型细胞因子介导RSV感染所诱发的气道炎症反应.Objective To investigate the role of CD4^＋T cells in airway inflammation induced by respiratory syncytial virus （RSV） infection. Methods Animal models of acute RSV infection were estab-lished. Lung tissues were stained with hematoxylin and eosin （HE） to observe histopathological changes. Total number of CD4^＋T cells and the number of CD4^＋T cells secreting Th1/Th2 cytokines （IFN-γ, IL-4, IL-5 and IL-13） in spleen were detected by flow cytometry. Adoptive transfer of CD4^＋T cells was performed to identify the role of CD4^＋T cells in RSV-induced airway inflammation. Results RSV infection increased the total number of splenic CD4^＋T cells,particularly Th2-type CD4^＋T cells. The absolute numbers of IL-4/IL-5/IL-13-secreting CD4^＋T cells were increased significantly after RSV infection. Furthermore, adoptive transfer of CD4^＋T cells into BALB/c mice not only promoted the infiltration of mononuclear cells in lung,but also enhanced the secretion of Th2 cytokines during RSV infection. Conclusion CD4^＋T cells are involved in RSV-induced airway inflammation through secreting Th2 cytokines.

关 键 词：呼吸道合胞病毒 CD4^+T细胞 气道炎症 

分 类 号：R392[医药卫生—免疫学]