16S rDNA克隆文库方法分析中华通草蛉共生细菌组成  

Analysis of Endophytic Symbiotic Bacterial Composition in Chrysoperla sinica(Tjeder) Adults with 16S rDNA Clone Library

作　　者：赵辉 张帅 雒珺瑜[2] 张利娟 王爱英[1] 崔金杰[2] 

机构地区：[1]石河子大学生命科学学院,石河子832000 [2]中国农业科学院棉花研究所/棉花生物学国家重点实验室,安阳455000

出　　处：《中国生物防治学报》2017年第6期849-856,共8页Chinese Journal of Biological Control

基　　金：转基因生物新品种培育重大专项(2016ZX08011-002)

摘　　要：中华通草蛉chrysoperla sinica(Tjeder)是农田生态系统中重要的捕食性天敌,为探索内生菌与宿主取食和生殖生理关系,本研究采用16S r DNA克隆文库的方法探究了中华通草蛉成虫内生菌的组成,分析了雌、雄成虫体内内生菌菌群结构是否存在差异。研究结果表明,中华通草蛉内生菌主要分3大类群:γ变形菌γ-Proteobacteria、α变形菌α-Proteobacteria和β变形菌β-Proteobacteria。按照属划分,中华通草蛉成虫共生细菌主要包含沃尔巴克氏体Wolbachia sp.、立克次氏体Rickettsia sp.、肠杆菌属Enterobacter sp.、免培养细菌Methyloversatilis sp.、沙雷氏菌Serratieae sp.、泛生菌属Pantoea sp.和欧文氏菌Erwinia sp.。仅在雌虫中存在Cosenzaea sp.,雄虫中存在醋酸杆菌属Acetobacter sp.、Asaia sp.、Rosenbergiella sp.、柠檬酸杆菌属Citrobacter sp.。得到中华通草蛉内生菌的优势菌群为沃尔巴克氏体Wolbachia pipientis,在雌、雄成虫中分别占23.2%和18.4%。结果证明中华通草蛉存在多种内生菌,雌、雄群体间存在差异。Chrysoperla sinica（Tjeder） is an important predator in farmland ecosystem. In order to explore the effects of endophytic bacteria on host feeding and reproductive physiology, the composition of endophyte in adults C. sinica was studied by the construction and analysis of a 16 S r DNA clone library. The results indicated that the bacterial could be divided into three major groups, i.e., Gamma Proteobacteria, Alpha Proteobacteria and Beta Proteobacteria. The endophyte in C. sinica adults are mainly composed of Wolbachia sp., Rickettsia sp.,Enterobacter sp., uncultured Methyloversatilis sp., Serratieae sp., Pantoea sp. and Erwinia sp. according to genus. Females and males differed in that Cosenzaea sp. occurs in female only, while Acetobacter sp., Asaia sp., Rosenbergiella sp., and Citrobacter sp. exist in males only. The dominant endophytes in C. sinica adults are Wolbachia pipientis, accounting for 23.3% in males and 18.4% in females. Therefore a diversity of bacteria exists in C. sinica, and there are sexual differences in composition of the bacteria.

关 键 词：中华通草蛉 16S rDNA文库 内生菌 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]