复方黄黛片灭活兔血清对人红白血病细胞增殖、凋亡及细胞周期的影响  

Effects of Inactivated Rabbit Serum Containing Compound Realgar and Natural Indigo Tablet on Proliferation,Apoptosis and Cell Cycle of Human Erythroleukemia Cells

作　　者：林双[1] 韩凌[1] 吴新忠[1] 冯梅[1] 卢爱丽[1] 吴远彬[1] 高红霞[1] 李达[1] 代喜平[1] 

机构地区：[1]广州中医药大学第二附属医院广东省中医院,广东广州510120

出　　处：《成都中医药大学学报》2017年第4期37-40,128,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(2013B021800231)

摘　　要：目的:探讨复方黄黛片(Compound Realgar and Natural Indigo Tablet,CRNIT)灭活兔血清对人红白血病(Human Erythroleukemia,HEL)细胞增殖、凋亡及细胞周期的影响;方法:制备复方黄黛片、羟基脲片、生理盐水三组灭活含药兔血清,用不同浓度的血清处理HEL细胞,通过CCK-8检测细胞增殖-毒性,通过Annexin V/PI双染色流式细胞分析检测细胞凋亡,Cell Cycle Staining Kit流式细胞分析检测细胞周期。结果:1.在一定浓度范围内,复方黄黛片灭活兔血清对HEL细胞增殖的抑制作用呈浓度依赖性,且强于羟基脲片组、生理盐水组(P<0.05);2.对比复方黄黛片灭活兔血清处理HEL细胞24 h、48 h抑制率,提示其抑制作用呈时间依赖性;3.在一定浓度范围内,复方黄黛片灭活兔血清对HEL细胞凋亡的促进作用呈浓度依赖性,且优于羟基脲片组和生理盐水组(P<0.05);4.经复方黄黛片灭活兔血清处理24 h,处于G1期的HEL细胞较对照孔增多。结论:复方黄黛片灭活兔血清能有效的抑制HEL细胞增殖,促进细胞早期凋亡,并影响HEL细胞周期。Objective： To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet （CRNIT） on proliferation, apoptosis and cell cycle of HEL cells. Methods： The inactivated rabbit serum was prepared with CRNIT, hydroxyurea tablet and saline respectively and different concentrations of serum were used in cell culture. Then the cell proliferation toxicity was detected by CCK-8, and the cell apoptosis was detected by Annexin, V/PI staining and flow cytometry. Finally the cell cycle were analyzed by Cell Cycle Staining Kit and flow eytometry. Results： 1. In certain range of serum concentration, the inhibitory effect on HEL cell proliferation was in a concentration-dependent manner in the CRNIT group, which was stronger than hydroxyurea tablet group and saline group （ P 〈 0. 05 ） ; 2. Comparing the inhibition rate of the HEL cells which were cultured with inactivated rabbit serum containing CRNIT at 24 h and 48 h, the inhibitory effect on HEL cells was in a time-dependent manner; 3. In certain range of serum concentration, the promoting effect on HEL cell apoptosis was in a concentration-dependent manner in the CRNIT group, which was stronger than hydroxyurea tablet group and saline group （ P 〈 0. 05 ） ; 4. HEL cell number in G1 period increased significantly than the control well when it was cultured with inactivated rabbit serum containing CRNIT for 24 h. Conclusion： The inactivated rabbit serum containing CRNIT could effectively inhibit the proliferation, promote the early apoptosis and affect the cell cycle of HEL cells.

关 键 词：HEL细胞 复方黄黛片 羟基脲 灭活血清 

分 类 号：R289[医药卫生—方剂学] R557[医药卫生—中药学]