细胞周期蛋白A1对SKM-1细胞增殖的影响及其在骨髓异常增生综合征中的作用  

Effects of Cyclin A1 on the Proliferation of SKM-1 Cells and Its Potential Role in Myelodysplastic Syndrome

作　　者：丁雪 程雅琳 高海成 卢文艺[1] 张瑶 贾晋松[1] 

机构地区：[1]北京大学人民医院北京大学血液病研究所,北京100044 [2]北京大学国际医院血液科,北京102206

出　　处：《中国实验血液学杂志》2017年第6期1733-1737,共5页Journal of Experimental Hematology

基　　金：北京市自然科学基金(7122190);教育部留学回国人员科研启动基金(教司留-贾晋松)

摘　　要：目的:通过小干扰RNA(siRNA)下调Cyclin A1的表达,观察其对骨髓增生异常综合征(MDS)细胞系SKM-1细胞增殖的影响并探讨其作用机制。方法:采用阳离子脂质体转染方法,在SKM-1细胞中转染Cyclin A1的siRNA;转染48 h后收集细胞,应用Western blot及RT-PCR测定转染效率;应用CCK-8方法检测细胞增殖,Western blot及RT-PCR检测Cyclin A1基因沉默前后CDK2、RUNX1、SRSF2基因表达的水平。结果:转染Cyclin A1特异性siRNA 48 h后,Cyclin A1蛋白及mRNA表达水平显著下降(P<0.01)。下调Cyclin A1后,SKM-1细胞的增殖受抑,CDK2、RUNX1及SRSF2基因的蛋白及mRNA表达量均下调(P<0.05)。结论:Cyclin A1表达下调后,SKM-1细胞的增殖受到抑制,表明Cyclin A1可能是MDS治疗的潜在靶点之一。Objective： To investigate the effects of Cyclin A1 on the proliferation of SKM-1 cells and its underlying role in myelodysplastic syndrome（ MDS）. Methods： Cyclin A1 was knocked down with its small interfering RNA（ siRNA）.The efficiency of siRNA transfection was measured by Western blot and RT-PCR. Then the proliferation of SKM-1 cells and the expression of CDK2,RUNX1 and SRSF2 with and without knockdown of Cyclin A1 recorded and analysed respectively.Results： Cyclin A1 was knocked down by siRNA after transfected for 48 h. The kncokdown of Cyclin A1 inhibited the proliferation of SKM-1 cells and down-regulated the expression of CDK2,RUNX1 and SRSF2,and these effects were at least partially mediated through RUNX1 and SRSF2 signaling pathway. Conclusion： Cyclin A1 plays an important role in the proliferation of SKM-1 cells. These findings provide new insights into the pathogenesis of MDS,and it may be a potential target in the treatment of MDS.

关 键 词：细胞周期蛋白A1 骨髓增生异常综合征 SKM-1细胞 

分 类 号：R551.3[医药卫生—血液循环系统疾病]