p15、DAPK、SOCS1和FHIT 4基因甲基化联合检测在骨髓增生异常综合征早期诊断及预后评估中的价值  

Clinical Value of p15,DAPK,SOCS1 and FHIT Genes Combined Detection in the Early Diagnosis and Prognosis Evaluation of Myelodysplastic Syndrome

作　　者：邓春阳[1] 陈双[1] 江明[1] 陈蓉[1] 尼罗帕尔.吐尔逊 王欢[1] 郝建萍[1] 

机构地区：[1]新疆医科大学第一附属医院血液病中心,新疆乌鲁木齐830054

出　　处：《中国实验血液学杂志》2017年第6期1751-1755,共5页Journal of Experimental Hematology

基　　金：新疆维吾尔自治区自然科学基因(2015211C046)

摘　　要：目的:探讨p15、DAPK、SOCS1和FHIT 4基因甲基化联合检测在骨髓增生异常综合征(MDS)早期诊断与预后评估中的价值。方法:应用甲基化特异性PCR(MSP)对67例MDS患者骨髓进行上述4种基因甲基化检测,分析4个基因联检在MDS患者早期诊断及预后评估中的价值。结果:67例MDS患者p15、DAPK、SOCS1和FHIT 4个基因甲基化率分别为37.3%、35.8%、47.8%和52.2%,较对照组显著增高(P<0.05),4个基因单检诊断MDS的阳性符合率分别为37.3%、35.8%、47.8%和52.2%,而4个基因联合检测诊断MDS的阳性符合率为82.1%,较单一基因检查阳性符合率明显增高(P<0.001);相对高危组中≥2个基因甲基化共表达明显高于相对低危组(P<0.05)。MDS患者中位生存时间18(13.3,22.7)个月,相对低危组患者中位生存时间明显长于相对高危组[27(20.3,33.7)vs 9(5.9,12.1)个月](P<0.05)。在不同危险度患者中,随着表达基因数的增多,患者的生存时间呈明显缩短趋势(P<0.05)。结论:p15、DAPK、SOCS1和FHIT 4种基因联合检测有利于提高MDS患者的早期诊断和预后判断。Objective： To investigate the value of p15,DAPK,SOCS1 and FHIT genes combined detection in the early diagnosis and prognosis evaluation of patients with myelodysplastic syndrome（ MDS）. Methods： The methylation-specific PCR（ MSP） was used to detect the methylation of the above-mentioned 4 genes in 67 patients with MDS. The value of 4 gene combined detection in the early diagnosis and prognosis evaluation of patients with MDS was compared and anazlyzed.Results： The methylation rates of p15,DAPK,SOCS1 and FHIT genes in 67 patients with MDS were 37. 3%,35. 8%,47. 8% and 52. 2%,respectively,which were significantly higher than those in control group（ P〈0. 05）. The accordance rates of p15,DAPK,SOCS1 and FHIT single detection for diagnosis of MDS were 37. 3%,35. 8%,47. 8% and 52. 2%,respectively,meanswhile the accordance rate of above-mentioned 4 gene combined detection for diagnosis of MDS was 82.1%,which was significantly higher than that of single gene detection（ P〈0. 001）. The methylation of ≥2 genes in relatively high risk group was significantly higher than that in relatively low risk group（ P〈0. 05）. The median survival time of MDS patients was 18（ 13. 3,22. 7） months; the median survival time in relatively low risk group was significantly longer than that in relatively high risk group [27（ 20. 3,33. 7） months vs 9（ 5. 9,12. 1） months]（ P〈0. 05）. The survival time of MDS patients with different risks displayed the trend of shorting feature along with increasing of methylated genes（ P〈0.05）. Conclusion： The combined detection of above menthioned 4 genes can improve the accuracy of early diagnosis and prognosis evaluation for MDS patients.

关 键 词：骨髓增生异常综合征 P15 DAPK SOCS1 FHIT 联合检测 早期诊断 

分 类 号：R551.3[医药卫生—血液循环系统疾病]