应用基因芯片技术筛查针刺人迎穴对原发性高血压大鼠下丘脑基因表达谱的影响  

Effects of acupuncture at Renying (ST9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

作　　者：郭妍 石学敏[1] 赵瑞利[2] 芦娟[2] 马田 

机构地区：[1]天津中医药大学第一附属医院,300192 [2]北京中医药大学针灸推拿学院

出　　处：《环球中医药》2017年第12期1417-1423,共7页Global Traditional Chinese Medicine

基　　金：国家中医药管理局专项(201507001-08)

摘　　要：目的本实验从基因芯片层面探讨针刺人迎穴对自发性高血压大鼠(spontaneous hypertensive rats,SHR)下丘脑基因表达的效应差异。方法将20只SHR随机分为人迎组和模型组,另取10只WKY大鼠作为空白对照组。人迎组每日针刺人迎穴并施以捻转泻法,模型组和空白组不进行针刺干预,并对大鼠每四天测量血压一次,实验共28天。结束后取材进行基因芯片分析。结果空白组血压无明显变化,模型组血压与空白组相比显著升高(P<0.01);人迎组血压虽未降至正常,但与模型组相比,差异具有统计学意义(P<0.01)。芯片结果显示:不同组别之间显示出基因表达的差异性。与空白组相比,模型组的数据分析显示有129个基因上调,243个基因下调;与模型组比较,人迎组有167个基因上调,188个基因下调。结论针刺人迎穴对SHR有明显的降压效应,对大鼠下丘脑的基因表达有一定影响,其机制可能与针灸抗高血压原理有关。Objective To explore the variance in gene profiles of hypothalamus in SHR by acupuncture at Renying （ST9） from the perspective of gene chip. Methods Twenty Spontaneously Hypertensive Rats（SHR） were randomly divided into Renying （ST9） group and model control group, and ten WKY rats were used as blank group. The acupuncture intervention was applied to Renying group everyday. The model group and normal group rats received no treatment. The blood pressure was measured every four days, the experiment lasted for 28 days. Gene microarray was performed after completion of the study. Results There was no significant change in the blood pressure of the blank group, and the blood pressure of the model group was significantly higher than that of the blank group （ P〈0.01 ）. Compared with model control group, blood pressure of Renying （ ST9 ） group did not drop to normal, but the blood pressure was remarkably decreased （P〈0.05） , the difference was statistically significant （P〈0.01）. Gene microarray results showed differences in gene expression between different groups. Compared with the blank group, there were 129 genes up-regulated and 243 genes down-regulated of the model group; compared with the model group, there were 167 genes up-regulated and 188 genes down regulated of Renying （ST9） group. Conclusion Acupuncture at Renying（ ST9 ） has a significant hypotensive effect on SHR, and has a certain effect on the gene expression of hypothalamus in rats, and its mechanism may be related to the antihypertensive principle of acupuncture.

关 键 词：针刺 人迎穴 原发性高血压 下丘脑 基因芯片 

分 类 号：R245.3[医药卫生—针灸推拿学]