HPLC法测定烫伤油及烫伤膜中大黄素的含量  

HPLC Determination of Content of Emodin in Scald Oil and Scald Membrane

作　　者：李嵘[1] 吴健[2] 谭家风[1] 徐康康[1] 李燕[1] 

机构地区：[1]南京医科大学附属南京儿童医院,江苏南京210008 [2]南京农业大学,江苏南京210029

出　　处：《南京中医药大学学报》2002年第5期300-301,共2页Journal of Nanjing University of Traditional Chinese Medicine

摘　　要：目的　建立烫伤膜及烫伤油中大黄素的含量测定方法。方法　采用HPLC法 ,C18柱 ,流动相为甲醇 - 1%磷酸水溶液 ,检测波长为 2 5 4nm。结果　大黄素在 7.2 0min出峰 ,烫伤膜、烫伤油的平均回收率分别为 96 .37%和 97.9% ,RSD分别为 0 .6 4 %和 1.11%。结论　烫伤油改为膜剂后 ,其主要成分大黄素的含量高于原有油剂 ,此检测方法简单快捷、准确实用。OBJECTIVE To establish the method for the determination of the content of emodin in Scald Oil and Scald Membrane.METHOD HPLC method was used, with C 18 column, methanol-phosphoric acid water solution as the mobile phase and 254 nm wavelength.RESULT Emodin reached its peat at 7.2 min, the recovery rates of Scald Membrane and Scald Oil were 96.37% and 97.9% respectively and RSD were 0.64% and 1.11% respectively. CONCLUSION After Scald Oil changes into membrane form, the content of the main component emodin is higher than the oil form. This method of determination is quick, accurate and practical.

关 键 词：烫伤膜 烫伤油 大黄素 高效液相色谱法 含量 中药 测定 

分 类 号：R284.1[医药卫生—中药学]