硼硅酸盐生物玻璃浸提液对成骨细胞增殖活力及成骨信号通路功能的调节作用  

Regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function

作　　者：何晓辉[1] 陈武生[1] 庄一凡[1] 汪道清 王锡奕 庄鑫泓 

机构地区：[1]广东省普宁市中医医院骨科,广东普宁515300

出　　处：《海南医学院学报》2017年第21期2910-2912,2916,共4页Journal of Hainan Medical University

基　　金：广东省惠州市科技计划项目(2016Y154)~~

摘　　要：目的:研究硼硅酸盐生物玻璃浸提液对成骨细胞增殖活力及成骨信号通路功能的调节作用。方法:培养成骨细胞MG-63并分为硼硅酸盐组和对照组,分别用含有硼硅酸盐生物玻璃浸提液的培养基处理和不含浸提液的培养基处理。处理24h后,测定细胞的增殖活力值以及增殖活力标志分子、Wnt信号通路分子、PI3K/AKT信号通路分子的表达量。结果:处理24h后,硼硅酸盐组的MTT细胞活力值显著高于对照组,细胞中ALP、OC、OPN、COL-I、Runx2、Wnt1、Wnt3a、β-catenin、LRP5、LRP6、pPI3K、p-AKT、Bcl-2、BMP的蛋白表达量显著高于对照组。结论:硼硅酸盐生物玻璃浸提液能够通过激活Wnt通路和PI3K/AKT通路增强成骨细胞的增殖活力。Objective:To study the regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function.Methods:Osteoblasts MG-63 were cultured and divided into borosilicate group and control group that were treated with the culture medium containing borosilicate bioglass extract and the culture medium without extract respectively.After 24 hours of treatment,the cell proliferation activity as well as the expression of proliferation activity markers,Wnt signaling pathway molecules and PI3 K/AKT signaling pathway molecules was measured.Results:After24 hours of treatment,MTT cell viability of borosilicate group was significantly higher than that of control group,and ALP,OC,OPN,COL-I,Runx2,Wnt1,Wnt3 a,β-catenin,LRP5,LRP6,p-PI3 K,p-AKT,Bcl-2 and BMP protein expression in cells were significantly higher than those of control group.Conclusion:Borosilicate bioglass extract can enhance the proliferation activity of osteoblasts by activating Wnt pathway and PI3 K/AKT pathway.

关 键 词：成骨细胞 硼硅酸盐生物玻璃材料 增殖活力 信号通路 

分 类 号：R683[医药卫生—骨科学]