建立玉米幼胚高效再生体系的研究  

Study on the Establishment of High Efficiency Regeneration System of Maize

作　　者：李金红[1,2] 霍岩 付莉[3] 陶承光 史振声 

机构地区：[1]辽宁省农业科学院创新中心,辽宁沈阳110161 [2]沈阳农业大学特种玉米研究所,辽宁沈阳110866 [3]锦州医科大学食品学院,辽宁锦州121000

出　　处：《辽宁农业科学》2017年第6期1-5,共5页Liaoning Agricultural Sciences

基　　金：辽宁省企业博士后项目(137301);国家现代农业产业技术体系(CARS-06-01-06);科技部其他项目(2016YFD0101803)

摘　　要：为建立高效的玉米再生体系,以玉米幼胚为材料,研究了不同浓度的L-Pro、AgNO_3和2,4-D对愈伤组织诱导率的影响,同时还分析了不同浓度的玉米素和IBA对分化和生根的影响,结果表明,愈伤组织诱导率最佳的组合为基本培养基中添加L-Pro 700 mg/L、AgNO_30.1 mg/L和2、4-D 1.5 mg/L;在基本培养基中添加5 mg/L的玉米素可有效促进愈伤组织分化,再生芽和再生植株分化率分别达到97.96%和97.67%,在生根培养基中添加0.1 mg/L的IBA生根效果较好。To establish high-frequency regenration technique system for gene tranformation in maize,We studyed on callus induction rate in L-Pro、AgNO_3 and 2,4-D when immature embryo is used as a material; we also analyzed the effects of different concentrations of ZT and IBA hormone on differentiation and rooting,The results showed that the best callus induction rate is added to L-Pro 700 mg/L、AgNO_30. 1 mg/L and 2、4-D1. 5 mg/L in basci medium; regeneration buds rate and regeneration plants rate reached 97. 96% and 97. 67% respectively if the basice mediuim was added to 5 mg/L zeatin; The result of rooting was the best when basice meidum was added 0. 1 mg/L IBA as the exogenous hormone.

关 键 词：玉米 幼胚 愈伤诱导率 再生 

分 类 号：S513.035.3[农业科学—作物学]