山萘酚对H22荷瘤小鼠的体内抗肿瘤作用  

Antitumor effect of kaempferol on H22 tumor-bearing in mice in vivo

作　　者：叶丽香[1] 阮冠宇[2] 毛晓丹[2] 宋旭辉 唐立威 

机构地区：[1]福建医科大学福建省新药安全性评价中心,福州350122 [2]福建省妇幼保健院妇科肿瘤实验室,福州350001

出　　处：《中国临床药理学杂志》2017年第24期2603-2605,共3页The Chinese Journal of Clinical Pharmacology

基　　金：福建省自然科学基金资助项目(2016J01491);福建省卫计委青年课题基金资助项目(2015-1-16);福建省妇幼保健院课题基金资助项目(妇保院研15-07)

摘　　要：目的研究山萘酚对2种H22荷瘤小鼠模型体内抗肿瘤作用。方法建立H22腹水瘤和实体瘤小鼠模型,按照体重将模型小鼠随机分为5组:模型组,对照组和低、中、高3个剂量实验组。模型组给予泊洛沙姆188,对照组给予30mg·kg^(-1)环磷酰胺(CTX),低、中、高3个剂量实验组分别给予150,300,600mg·kg^(-1)山萘酚混悬液,各组采用灌胃给药,给药体积为20 mL·kg^(-1),连续给药8 d。末次给药24 h后,处死小鼠,记录H22腹水瘤小鼠腹水量和瘤重;计算肝系数(LI)、脾系数(SI)。结果在腹水瘤实验中,模型组、对照组与低、中、高3个剂量实验组的腹水量分别为(9.85±1.99),(2.28±2.74),(8.44±2.51),(5.91±2.29),(4.98±4.25)mL,与模型组比较,对照组与中高2个剂量实验组差异均有统计学意义(均P<0.05);模型组、对照组与低、中、高3个剂量实验组的LI分别为4.72±2.22,5.42±0.77,4.69±0.94,5.22±0.60,5.27±0.61,这5组的SI分别为0.49±0.33,0.62±0.19,0.51±0.13,0.49±0.14,0.41±0.14,组间比较差异均无统计学意义(均P>0.05)。在实体瘤实验中,模型组、对照组与低、中、高3个剂量实验组的瘤块重分别为(1.70±0.80),(0.73±0.51),(1.14±0.91),(1.07±0.34),(0.95±0.60)g,与模型组比较差异均有统计学意义(均P<0.05)。结论山萘酚对H22荷瘤小鼠具有一定的抗肿瘤作用,对肝和脾的影响较小。Objective To explore the effect of kaempferol on tumor growth, weight, liver index （LI） and spleen index （SI） in different models of H22 tumor- bearing mice. Methods The H22 ascitie and solid tumor- beating mice model were established, then mice were randomized into five groups, including model group, control group （cy- elophosphamide 30 mg·kg-1 ） , experimental -L, - M, - H groups （ kaempferol 150,300,600 mg· kg-1 ）. The samples were obtained in 24 h after continuous intragastrical administration of drug or solvent to mice for 8 d. The ascitie volume and tumor weight were measured. The liver index（LI） , spleen index （SI） were calculated. Results In H22 aseitic tumor- bearing mice, the ascitic volume of mice in model group, control group, experimental - L, - M, - H groups were （9.85 ± 1.99） , （2.28 ±2. 74） ,（8.44 ±2.51）, （5.91 ±2.29）,（4.98 ±4.25） mL; com- pared with model group, the difference in control group, experimental - M, - H groups was significantly （ all P 〈 0. 05 ）. LI and SI in above five groups were 4. 72 ± 2.22, 5.42 ±0. 77,4. 69 ± 0.94, 5.22± 0. 60, 5.27±0.61;0.49 ± 0.33,0,62 ± 0. 19,0.51 ±0. 13,0.49 ±0.14,0. 41 ±0. 14 with not significantly between five groups （all P 〉 0. 05 ）. In H22 solid tumor -bearing mice, the tumor weight of mice in model group, control group, experimental - L, - M, - H groups were （ 1.70 ± 0.80） , （0. 73±0. 51 ）, （ 1.14±0.91 ）, （ 1.07 ±0. 34）, （0. 95 ±0.60） g with significantly compared with model group（ all P 〈 0.05 ）. Conclusion The kaempferol had anti - tumor effects toward H22 tumor - bearing mice with less influence on liver and spleen.

关 键 词：山萘酚 H22肝癌 抗肿瘤 腹水瘤 实体瘤 

分 类 号：R28[医药卫生—中药学]