miR-206在卵巢颗粒细胞的表达调节及与多囊卵巢综合征的相关性  

Expression of miR-206 in ovarian granulosa cells and its correlation with polycystic ovary syndrome

作　　者：朱琳[1] 张晨霞[2] 戴洁[2] 谭洁[2] 吴群英[2] 凌静[2] 

机构地区：[1]江阴市人民医院中心实验室 [2]江阴市人民医院妇产科,江苏江阴214400

出　　处：《牡丹江医学院学报》2017年第6期17-19,37,共4页Journal of Mudanjiang Medical University

基　　金：江阴市社会发展科技计划项目/澄政科([2015]77号)

摘　　要：目的探讨miR-206在多囊卵巢综合征(PCOS)小鼠卵巢颗粒细胞中的表达,以及不同浓度的雄激素(双氢睾酮)和胰岛素对小鼠正常卵巢颗粒细胞中miR-206的表达调节,为研究多囊卵巢的发病机制提供理论依据。方法构建小鼠PCOS模型,分别提取PCOS小鼠卵巢颗粒细胞和健康小鼠卵巢颗粒细胞,应用实时荧光定量PCR方法检测miR-206在两组细胞中的表达。正常小鼠卵巢颗粒细胞体外培养48h后,分别用不同浓度的双氢睾酮和胰岛素刺激颗粒细胞24h后,应用实时荧光定量PCR方法检测miR-206的表达。结果 PCOS小鼠卵巢颗粒细胞中miR-206表达高于正常组(P<0.05)。高浓度双氢睾酮和胰岛素刺激的颗粒细胞中miR-206表达明显高于正常组(P<0.05)。结论 miR-206在PCOS小鼠卵巢颗粒细胞中的表达比正常组明显增加,高浓度双氢睾酮和胰岛素可能会导致miR-206表达增加,miR-206可能参与多囊卵巢发病的过程。Objective To investigate the expression of miR - 206 in the ovarian granulosa ceils of polycystic ovary syndrome （PCOS） mouse model. To investigate the effect of different concentrations of androgen （dihydrotestosterone） and insulin on the expression of miR - 206 in mouse normal ovarian granulosa cells, and to provide a theoretical basis for the study of the pathogenesis of polycystic ovary. Methods To construct the mouse PCOS model, and to extract PCOS mouse ovarian granulosa ceils and healthy mouse ovarian granulosa cells. The expression of miR - 206 in two groups was detected by Real - time PCR. Normal mouse granulosa cells were cultured in vitro for 48 h. After the different concentrations of DHT and insulin were stimulated granulosa cells for 24h, the expression of miR - 206 was detected by real - time PCR. Results The expression of miR - 206 in ovarian granulosa ceils of PCOS mice was higher than that in the normal group （ P 〈 0.05 ）. The expression of miR - 206 in granule cells stimulated with high concentration of dihydrotestosterone and insulin was significantly higher than the normal group （P 〈 0.05）. Conclusion The expression of miR - 206 in PCOS mouse granulosa ceils was higher than the normal group. The high concentrations of dihydrotestosterone and insulin may lead to increase the expression of miR - 206. MiR - 206 might be involved in the pathogenesis of polycystic ovary.

关 键 词：MIRNA 多囊卵巢 颗粒细胞 凋亡 miR-206 

分 类 号：R71[医药卫生—妇产科学]