促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化  

Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection

作　　者：苗莹莹 鲁明 赵俊强 

机构地区：[1]新乡医学院三全学院人体解剖学教研室 [2]新乡医学院三全学院教育技术中心,河南新乡453003

出　　处：《中国临床解剖学杂志》2017年第6期645-649,共5页Chinese Journal of Clinical Anatomy

基　　金：2017年河南省软科学研究计划课题(172400410495);国家自然科学基金资助项目(81600677);河南省医用组织再生重点实验室开放课题(KFKT16003)

摘　　要：目的观察注射促红细胞生成素(Erythropoietin,EPO)后,高脂喂养(high fat diet,HFD)的肥胖小鼠模型体重、脂肪含量变化以及白色脂肪组织、棕色脂肪组织中过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPAR γ)、解偶联蛋白1(uncoupling protein 1,UCP1)、β3肾上腺素能受体(β3 adrenoceptor,β3-AR)mRNA以及蛋白的表达变化,为EPO治疗肥胖的机制提供线索。方法 20只高脂喂养的C57BL/6J雄鼠随机分为对照组(HFD-Con)和EPO组(HFD-EPO)。4周后比较两组动物的体重,实时定量PCR方法检测皮下脂肪、附睾脂肪以及棕色脂肪中的PPAR γ、UCP1和β3-AR mRNA表达变化。结果 4周后,与对照组比较,EPO组体重(24.53±2.03)g,HFD-Con组体重为(31.78±1.68)g,P<0.001。HFD-EPO组动物的皮下脂肪与附睾脂肪重量均显著减少,P<0.001。HE染色结果显示,相较于HFD-Con组,HFD-EPO组动物的脂肪细胞减小。半定量PCR和Western blotting结果显示,皮下脂肪组织与附睾脂肪组织中的PPAR γ、UCP1、β3-AR mRNA以及蛋白的表达均无显著性改变,而棕色脂肪中的PPAR γ、UCP1、β3-AR的mRNA以及蛋白表达均显著增加。结论棕色脂肪中PPAR γ、UCP1、β3-AR表达变化可能与高脂喂养后注射EPO导致体重减轻存在一定的联系。Objective Erythropoietin（EPO） has been reported to have a beneficial effect on obesity.The aim of the study is to investigate the changes of body weight and fat content of EPO in high fat diet（HFD） mice, the expression of PPAR γ, UCP1, β3-AR mRNA in white adipose tissue（WAT） and brown adipose tissue（BAT）. Methods 20 HFD-fed C57 BL/6 J male mice were randomly divided into control group（HFD-Con） and EPO group（HFD-EPO）. The expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous fat, epididymal fat and brown fat were detected by real-time quantitative RT-PCR. Results Compared with the control group, the body weight of the EPO mice was（24.53±2.03） g and the control group was（31.78±1.68） g. The weight of subcutaneous fat and epididymal fat in HFD-EPO group were significantly decreased. HE staining showed that the size of adipocytes of HFD-EPO group was decreased compared with HFD-Con group. Quantitative real-time PCR results showed that the expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous adipose tissue and epididymal adipose tissue did not change significantly, while the expression of PPAR γ, UCP1 and β3-AR in brown fat were significantly increased. Conclusion The expression of PPAR γ, UCP1 and β3-AR in brown fat may be related to the weight loss caused by EPO injection after high fat diet.

关 键 词：高脂喂养 促红细胞生成素 过氧化物酶体激活增殖受体γ 解偶联蛋白1 Β3肾上腺素能受体 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R354[医药卫生—基础医学]