中药干预对糖尿病肾病大鼠蛋白尿及炎性细胞因子表达的影响  

Effect of traditional Chinese medicine on the expression of proteinuria and inflammatory factors in diabetic kidney disease rats

作　　者：安金龙[1] 张文军[1] 金仲达[1] 俞仲贤[1] 周丽娜[1] 张雪峰[1] 

机构地区：[1]江苏省常熟市第一人民医院中医科,215500

出　　处：《国际中医中药杂志》2017年第12期1103-1107,共5页International Journal of Traditional Chinese Medicine

基　　金：江苏省中医药科技项目（YB2015109）

摘　　要：目的 通过建立糖尿病肾病（diabetic kidney disease,DKD）大鼠模型,观察中药复方对DKD大鼠蛋白尿及炎性细胞因子影响.方法 健康雄性SD大鼠49只,以随机数字表法分为正常组、模型组、中药组、对照组.采用左下腹腔一次性注射STZ诱导DKD模型.正常组和模型组给予等量0.5%羧甲基纤维素钠,中药组给予中药复方药液,对照组给予厄贝沙坦药液,给药容量为10ml/kg,1次/d,连续灌胃8周.观察大鼠肾指数（肾脏重量/大鼠体重）、FPG、IL-6、尿微量白蛋白（urinary albumin,mAlb）、单核细胞趋化蛋白-1（monocyte chemotactic protein-1,MCP-1）、TGF-β等变化.结果 与模型组比较,中药组FPG[（9.96±3.73）mmol/L比（17.16±5.75）mmol/L]下降（P〈0.05）,mAlb[（11.73±4.01）mg/L比（19.57±8.38）mg/L]降低（P〈0.05）;IL-6水平[（58.87±14.69）ng/L比（85.22±12.16）ng/L]下降（P〈0.05）,MCP-1含量[（1.52±0.86）ng/L比（3.17±1.26）ng/L]下降（P〈0.05）.结论 中药可降低DKD模型大鼠的FPG、mAlb,清除血清炎性细胞因子,疗效与厄贝沙坦片相当.Objective To observe the effect of traditional Chinese medicine on proteinuria and inflammatory factors on the model of diabetic kidney disease rats. Methods The male SD rats were divided into the blank group, model group, treatment group, control group. The diabetic kidney disease model was induced by using the left inferior abdominal cavity. After eight weeks, we observed the changes of the rat＆#39;s weight/kidney weight, blood sugar, IL-6, urine microalbumin, MCP-1 and TGF-β. Results Compared with the model group,the blood glucose（9.96 ±3.73 mmol/L vs.17.16 ±5.75 mmol/L）and Proteinuria（11.73 ±4.01 mg/L vs.19.57 ± 8.38 mg/L,P〈0.05） of the treatment group significantly decreased （P〈0.05）. Compared with the model group,the level of IL-6（58.87 ±14.69 ng/L vs.85.22 ±12.16 ng/L）and the MCP-1（1.52 ±0.86 ng/L vs.3.17 ± 1.26 ng/L）of the treatment group were significantly lower （P〈0.05）. Conclusions The Chinese medicine can reduce the FPG, proteinuria and the removal of serum inflammatory factors in diabetic kidney disease.

关 键 词：中药 糖尿病肾病 蛋白尿 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]