健脾化痰方对裸鼠前胃癌肝转移作用及其机制的研究  

The research of Jianpi-Huatan decoction resisting MFC hepatic metastasis and its mechanism

作　　者：许瑶[1] 许尤琪[1] 沈明勤[2] 

机构地区：[1]南京中医药大学第二附属医院肿瘤科,210017 [2]江苏省中医药研究院药理毒理实验室,南京,210028

出　　处：《国际中医中药杂志》2017年第12期1108-1112,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的 探讨健脾化痰方对裸鼠前胃癌（murine foregastric carcinoma,MFC）肝转移的影响及其作用机制.方法 将MFC细胞接种于裸鼠脾脏,建立裸鼠肝转移模型后用随机数字表法分为模型组、5-fu注射液组及健脾化痰方高、中、低剂量组,健脾化痰方高、中、低剂量组分别灌胃80、40、20g/kg的健脾化痰方煎剂,5-fu组腹腔注射60mg/kg 5-fu注射液,模型组灌胃等体积生理盐水,1次/d,共4周.实验结束时计算裸鼠体重、脾脏瘤重,评估肝脏转移情况,用RT-PCR法、免疫组织化学法分别检测P53、Bcl-2基因和相应蛋白表达的情况.结果 与模型组比较,健脾化痰方中剂量组、高剂量组裸鼠体重[（21.40±1.43）g、（21.70±1.02）g比（20.37±1.17）g]明显增加（P〈0.05）,脾脏瘤重[（0.26±0.13）g、（0.16±0.05）g比（0.63±0.17）g]明显减轻（P〈0.05）;健脾化痰方中、高剂量组裸鼠肝脏表面转移瘤数目较少（P〈0.05）;与模型组比较,健脾化痰方低、中、高剂量组P53 mRNA表达[（8.32±0.38）、（5.42±0.45）、（3.09±0.26）比（9.67±1.31）]、Bcl-2 mRNA表达[（4.65±0.61）、（3.22±0.21）、（2.49±0.19）比（5.32±0.42）]均降低（P〈0.05）;健脾化痰方低、中、高剂量组脾脏接种瘤组织中P53蛋白的阳性表达率[（76.11±5.23）、（45.20±3.77）、（23.11±3.14）比（81.63±5.01）]均低于模型组（P〈0.05）,Bcl-2蛋白[分别为（58.67±5.27）、（32.00±3.13）（19.00±2.54）比（63.00±4.10）]均低于模型组（P〈0.05）.结论 健脾化痰方可抑制裸鼠前胃癌移植瘤的生长及肝转移,下调P53、Bcl-2基因和相应蛋白的表达.Objective To explore the research of Jianpi-Huatan decoction resisting the nude mouse MFC hepatic metastasis and its mechanism. Methods MFC cells inoculation in nude mice spleen, establishing nude mice hepatic metastasis model, which are divided into model group, 5-fu injection group, Jianpi-Huatan decoction high, medium and low dose group according to radom number table. Mice in high, medium and low dose Jianpi-Huatan decoction groups were adiminstrated with 80,40 and 20 g/kg Jianpi-Huatan decoction,in 5-fu groups were adiminstrated by intraperitoneal injection with 60mg/kg 5-fu injection and in model groups with Physiological saline once a day for 4 consecutive weeks. After the end, calculate the nude mice weight, spleen tumor weight and evaluation of hepatic metastases. And immune histochemical method and RT-PCR method is applied to detect tumor tissue of the expression of P53, Bcl-2 protein and mRNA. Results Compared with model group, Jianpi-Huatan decoction high and medium dose group can obviously increase body weight[（21.40 ± 1.43）g, （21.70 ± 1.02）g vs.（20.37 ± 1.17）g] and reduce the in situ tumor weight [（0.26 ±0.13）g,（0.16 ±0.05）g vs.（0.63 ±0.17）g]and the number of liver metastases;the mRNA levels of P53 and Bcl-2 （8.32 ±0.38,5.42 ±0.45,3.09 ±0.26 vs.9.67 ±1.31）and（4.65 ±0.61,3.22 ±0.21,2.49 ±0.19 vs.5.32 ±0.42） were decreased in low,medium and high dose Jianpi-Huatan decoction groups;P53（76.11 ±5.23,45.20 ±3.77, 23.11 ± 3.14 vs.81.63 ± 5.01）and Bcl-2（58.67 ± 5.27,32.00 ± 3.13,19.00 ± 2.54 vs.63.00 ± 4.10）levels were down-regulated in each dose of Jianpi-Huatan decoction group.Conclusions Jianpi-Huatan decoction can restrain nude mouse transplantation tumor growth and hepatic metastasis, which related to the cut of the expression of P53, Bcl-2 gene and protein.

关 键 词：基因 BCL-2 基因 p53 健脾化痰方 裸鼠前胃癌 肝转移 GENES BCL-2 GENES p53 

分 类 号：R285.5[医药卫生—中药学]