胰岛素样生长因子结合蛋白-3在原代培养的阴茎海绵体平滑肌细胞中的表达及定位  

Expression and localization of insulin-like growth factor binding protein-3 in the corpus carvernosum smooth muscle cells

作　　者：蒲小勇[1] 周香雪[2] 肖恒军[3] 林楚琪 彭伟华[1] 毕学成[1] 李东[1] 刘久敏[1] 

机构地区：[1]广东省医学科学院,广东省人民医院泌尿外科,广州510080 [2]中山大学附属第一医院东院神经内科,广州510700 [3]中山大学附属第三医院泌尿外科,广州510600

出　　处：《中国性科学》2017年第12期7-10,共4页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金资助(81570691,81270855);广东省中医药局科研项目(20161001);广州市科技计划项目(2014J4100041)

摘　　要：目的:观察胰岛素样生长因子结合蛋白-3(insulin-like growth factor binding protein 3,IGFBP-3)在阴茎海绵体平滑肌细胞(corpus cavernosum endothelial cells,CCSMCs)中的表达及定位情况。方法:原代培养大鼠CCSMCs并鉴定,RT-PCR检测IGFBP-3基因mRNA表达,用荧光免疫细胞化学法检测IGFBP-3在CCSMCs中的定位及蛋白表达。结果:RT-PCR能够检测到IGFBP-3 802bp特异性条带的表达,荧光免疫细胞化学法可见IGFBP-3蛋白表达并同时定位于CCSMCs胞质和胞核中。结论:CCSMCs可表达IGFBP-3基因,其蛋白定位于胞质和胞核,可能是ED治疗的新靶点。Objectives： To observe the expression and localization of insulin-like growth factor binding protein-3（ IGFBP-3） in the corpus carvernosum smooth muscle cells（ CCSMCs） of rat. Methods： The CCSMCs were primary cultured and identified. IGFBP-3 mRNA was detected by RT-PCR. The protein expression and the localization were confirmed by indirect immunofluorescence staining. Results： The targeted specific802 bp bands were detected in the cells by RT-PCR. Indirect immunofluorescence staining revealed that the expression of IGFBP-3 in both the cytoblast and cytoplasma in CCSMCs. Conclusion： The mRNA and protein of IGFBP-3 are expressed in CCSMCs. The expression of the protein is located in both cytoblast and cytoplasm in the cells and it may be a potential new target for treatment of erectile dysfunction（ ED）.

关 键 词：胰岛素样生长因子结合蛋白-3 细胞培养 阴茎海绵体平滑肌细胞 

分 类 号：R3[医药卫生—基础医学]