病理性瘢痕组织中TSG-6基因表达与细胞凋亡、胶原代谢的相关性研究  

The correlation of TSG-6 expression with apoptosis and collagen metabolism in pathological scar tissue

作　　者：卫东[1] 杜丽萍[1] 张家建[1] 

机构地区：[1]四川省人民医院.四川省医学科学院.电子科技大学附属医院整形美容科,四川成都610072

出　　处：《海南医学院学报》2017年第20期2884-2886,2890,共4页Journal of Hainan Medical University

基　　金：成都市惠民技术研发项目(2014-HM01-00274-SF)~~

摘　　要：目的:研究病理性瘢痕组织中肿瘤坏死因子α刺激基因-6(TSG-6)基因表达与细胞凋亡、胶原代谢的相关性。方法:选择在医院接受手术切除的病理性瘢痕组织以及因四肢外伤接受手术切除的正常皮肤组织,检测组织中TSG-6的mRNA表达量以及凋亡基因、胶原代谢指标的蛋白含量。结果:病理性瘢痕组织中TSG-6的mRNA表达量以及Smac、p53、Cyt-C、IGF-1、bFGF的蛋白含量显著低于正常皮肤组织,Livin、PCNA、Col-I、Col-III、TGF-β1的蛋白含量显著高于正常皮肤组织;TSG-6高表达的病理性瘢痕组织中Livin、PCNA、Col-I、Col-III、TGF-β1的含量低于TSG-6低表达的病理性瘢痕组织,Smac、p53、Cyt-C、IGF-1、bFGF的含量显著高于TSG-6低表达的病理性瘢痕组织。结论:病理性瘢痕组织中TSG-6基因的低表达能够抑制细胞凋亡、促进胶原合成代谢,进而加速瘢痕的形成。Objective：To study the correlation of tumor necrosis factor-αstimulated gene-6（TSG-6）expression with apoptosis and collagen metabolism in pathological scar tissue.Methods：Surgically removed pathological scar tissue and the normal skin tissue removed due to extremity trauma in Sichuan Provincial People＇s Hospital between June 2014 and February 2017 were selected to detect the mRNA expression of TSG-6 as well as the protein levels of apoptosis genes and collagen metabolism indexes.Results：TSG-6 mRNA expression as well as Smac,p53,Cyt-C,IGF-1 and bFGF protein levels in pathological scar tissue was significantly lower than those in normal skin tissue while Livin,PCNA,Col-I,Col-III and TGF-β1 protein levels were significantly higher than those in normal skin tissue;Livin,PCNA,Col-I,Col-III and TGF-β1 protein levels in pathological scar tissue with higher TSG-6 expression were lower than those in pathological scar tissue with lower TSG-6 expression while Smac,p53,Cyt-C,IGF-1 and bFGF protein levels were significantly higher than those in pathological scar tissue with lower TSG-6 expression.Conclusion：The low expression of TSG-6 in pathological scar tissue can inhibit apoptosis and promote collagen anabolism to accelerate scar formation.

关 键 词：病理性瘢痕 肿瘤坏死因子α刺激基因-6 凋亡 胶原 

分 类 号：R619.6[医药卫生—外科学]