膀胱癌病灶内miR-21的表达量及其对癌细胞侵袭、血管新生的影响  

miR-21 expression in bladder cancer lesion and its effect on cancer cell invasion and angiogenesis

作　　者：王亚辉 段建敏[1] 杜昌国 叶明宝 董治春 燕群峰 

机构地区：[1]兰州大学第二医院泌尿外科,甘肃兰州730000 [2]陕西省咸阳市第一人民医院泌尿外科,陕西咸阳712000

出　　处：《海南医学院学报》2017年第23期3278-3281,共4页Journal of Hainan Medical University

基　　金：陕西省卫生厅科研基金项目(2010H21)~~

摘　　要：目的:研究膀胱癌病灶内miR-21的表达量及其对癌细胞侵袭、血管新生的影响。方法:选择2015年2月~2017年6月期间在我院手术切除的94例膀胱癌患者作为研究对象,收集膀胱癌病灶和癌旁病灶适量并检测miR-21的表达量及侵袭基因、血管新生基因的mRNA表达量。结果:膀胱癌病灶内miR-21的表达量显著高于癌旁病灶;膀胱癌病灶内CDH13、CBX7的mRNA表达量显著低于癌旁病灶,TRAP-1、KPNA2、TRPM8、EDIL3、HGF、VEGF、Epha2的mRNA表达量显著高于癌旁病灶。miR-21高表达的膀胱癌病灶内CDH13、CBX7的mRNA表达量显著低于miR-21低表达的膀胱癌病灶,TRAP-1、KPNA2、TRPM8、EDIL3、HGF、VEGF、Epha2的mRNA表达量显著高于miR-21低表达的膀胱癌病灶。结论:膀胱癌病灶内高表达的miR-21能够促进癌细胞的侵袭以及病灶内的血管新生。Objective:To study the miR-21 expression in bladder cancer lesion and its effect on cancer cell invasion and angiogenesis.Methods:Total of 94 patients with bladder cancer who received surgical resection in our hospital between February 2015 and June 2017 were selected as the research subjects,and proper amount of bladder cancer lesion and adjacent lesion were collected to detect the expression of miR-21 as well as the mRNA expression of invasion genes and angiogenesis genes.Results:miR-21 expression in bladder cancer lesion was significantly higher than that in adjacent lesion;CDH13 and CBX7 mRNA expression in bladder cancer lesion were significantly lower than those in adjacent lesion while TRAP-1,KPNA2,TRPM8,EDIL3,HGF,VEGF and Epha2 mRNA expression were significantly higher than those in adjacent lesion.CDH13 and CBX7 mRNA expression in bladder cancer lesion with high miR-21 expression were significantly lower than those in bladder cancer lesion with low miR-21 expression while TRAP-1,KPNA2,TRPM8,EDIL3,HGF,VEGF and Epha2 mRNA expression were significantly higher than those in bladder cancer lesion with low miR-21 expression.Conclusions:The high expression of miR-21 in bladder cancer can promote the invasion of cancer cells and the angiogenesis in the lesion.

关 键 词：膀胱癌 MIR-21 侵袭 血管新生 

分 类 号：R757.14[医药卫生—皮肤病学与性病学]