Geminiviruses and their application in biotechnology  

作　　者：YANG Qiu-ying DING Bo ZHOU Xue-ping 

机构地区：[1]State Key Laboratory for Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193, P.R.China

出　　处：《Journal of Integrative Agriculture》2017年第12期2761-2771,共11页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31672006 and 31390422)

摘　　要：Being a major class of single-stranded DNA viruses, geminiviruses are mostly studied due to their catastrophic infectious effect on crops. These DNA viruses are characteristic for their ability in quickly multiplying viral genetic materials without integrating into the genome of plants, which makes them ideal for developing viral vectors for plants bioengineering. Geminivirus-derived vectors can be classified into expression vectors and virus-induced gene silencing（VIGS） vectors. Details of the design, construction, application and improvements of these geminivirus vectors are summarized and discussed.Being a major class of single-stranded DNA viruses, geminiviruses are mostly studied due to their catastrophic infectious effect on crops. These DNA viruses are characteristic for their ability in quickly multiplying viral genetic materials without integrating into the genome of plants, which makes them ideal for developing viral vectors for plants bioengineering. Geminivirus-derived vectors can be classified into expression vectors and virus-induced gene silencing（VIGS） vectors. Details of the design, construction, application and improvements of these geminivirus vectors are summarized and discussed.

关 键 词：GEMINIVIRUS virus-based vectors expression vectors VIGS vectors BIOTECHNOLOGY 

分 类 号：S432.41[农业科学—植物病理学]