碳/碳复合材料表面掺镁羟基磷灰石生物涂层的体外性能  

In vitro properties of magnesium doped hydroxyapatite coating on the surface of carbon/carbon composites

作　　者：倪昕晔 熊信柏[2] 尤瑞金[3] 吴兴盛 李万帅 

机构地区：[1]南京医科大学附属常州第二人民医院,江苏省常州市213003 [2]深圳大学材料学院,广东省深圳市518060 [3]解放军第180医院,福建省泉州市362000 [4]台湾新光吴火狮纪念医院,台北11101 [5]常州杰傲医学检验所有限公司,江苏省常州市213000

出　　处：《中国组织工程研究》2017年第34期5443-5448,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助(51672178);常州市社会发展项目(CJ20160029);江苏省自然科学基金(BK20151181);常州市卫生高层次人才资助项目(2016CZLJ004)~~

摘　　要：背景:前期研究利用电磁感应沉积法在碳/碳复合材料表面成功制备了含镁的羟基磷灰石涂层,分析了不同感应沉积时间对含镁羟基磷灰石涂层形貌的影响。目的:进一步探讨碳/碳复合材料表面掺镁羟基磷灰石涂层的体外性能。方法:在碳/碳复合材料表面分别制备羟基磷灰石涂层(对照组)与掺镁羟基磷灰石涂层(实验组)。①体外溶解性:将含两种涂层的碳/碳复合材料分别浸泡于DMEM低糖培养基中,检测浸泡前后的涂层表面形貌、离子浓度及涂层结合力;②细胞相容性:将含两种涂层的碳/碳复合材料分别与小鼠骨髓间充质干细胞共培养6 d,扫描电镜观察细胞增殖。结果与结论:①浸泡1 d后,两组涂层表面已有沉积物形成,实验组涂层表面沉积物多于对照组;浸泡6 d后,实验组涂层表面被沉积物完全覆盖,对照组涂层表面沉积物还有部分空隙存在。浸泡6 d后,两种涂层表面沉积物含有钙和磷元素,且实验组涂层表面新生涂层的镁离子含量明显高于对照组;②浸泡6 d后,对照组涂层表面的钙、磷、镁离子浓度逐渐降低;实验组涂层表面的钙、磷离子浓度逐渐降低,镁离子浓度逐渐升高,且实验组涂层表面镁离子浓度高于对照组(P<0.05);③实验组浸泡前及浸泡6 d后的涂层结合力均高于对照组(P<0.05);④实验组表面骨髓间充质干细胞的增殖能力强于对照组;⑤结果表明,掺镁羟基磷灰石涂层具有较好的结合力与细胞相容性。BACKGROUND： In the early study, hydroxyapatite coatings containing magnesium （Mg-HA） were successfullyprepared on the carbon/carbon composite surface by electromagnetic induction deposition. The effects of differentinduction deposition time on the morphology of Mg-HA coating were analyzed.OBJECTIVE： To investigate the in vitro properties of Mg-HA coating on the carbon/carbon composites.METHODS： HA （control group） and Mg-HA （experimental group） coating were prepared on the surface ofcarbon/carbon composites. （1） In vitro solubility： Carbon/carbon composites with different coatings were immersed inlow-glucose DMEM medium, and surface morphology, ion concentration, and coating adhesion were tested beforeand after immersion. （2） Cytocompatibility： Carbon/carbon composites with different coatings were respectivelycultured with mouse bone marrow mesenchymal stem cells for 6 days, and cell proliferation was measured byscanning electron microscope.RESULTS AND CONCLUSION： （1） One day after soaking, sediments formed on the coating surface in both groups,but there were more sediments in the experimental group than the control group. Six days after soaking, thesediments completely covered the coating surface in the experimental group, while there were still some gaps in thecontrol group; Ca and P were both detected on the coating surface in the two groups, and the Mg content on thecoating surface was obviously higher in the experimental group than the control group. （2） Six days after soaking, theconcentrations of Ca, P and Mg in the control group continued to fall; in the experimental group, the concentrations ofCa and P were also reduced gradually, but the Mg concentration arose gradually. Moreover, the Mg concentrationwas significantly higher in the experimental group than the control group （P 〈 0.05）. （3） The bonding strength of thecoating in the experimental group was higher than that in the control group before and after 6 days soaking. Insummary, the Mg-HA coating has

关 键 词：羟基磷灰石类 镁 组织工程 生物材料 材料相容性 碳/碳复合材料 羟基磷灰石 涂层 结合力 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]