细菌纳米纤维素/壳聚糖复合管制备及其作为小径人工血管潜力的评价  

Preparation and evaluation of bacterial nano-cellulose/chitosan composite tubes as potential small-diameter vascular grafts

作　　者：李雪 唐敬玉[1] 包露涵 陈琳[1] 洪枫[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海市201620

出　　处：《中国组织工程研究》2017年第34期5467-5473,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(51373031);教育部新世纪优秀人才支持计划(NCET-12-0828);上海市科委"科技创新行动计划"项目(15520720800);中央高校基本科研业务费专项资金资助项目~~

摘　　要：背景:目前临床上仍旧缺乏人工血管用于小口径血管(<6 mm)的替换。目的:探究细菌纳米纤维素/壳聚糖复合管作为小口径人工血管的可行性。方法:将培养制备的细菌纳米纤维素管分别与0.5%,1%,2%的壳聚糖复合,制备细菌纳米纤维素/壳聚糖复合管。对这些管材进行宏观形态、微观结构、密度、持水量、爆破压、机械力学性能以及血液相容性和细胞相容性等表征。结果与结论:①与细菌纳米纤维素管相比,细菌纳米纤维素/壳聚糖复合管的水渗透性减小,密度和持水能力均增大,轴向和径向力学性能增强,但是延展性和弹性降低;②对于血液相容性而言,细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管的溶血率均能满足医用材料要求,但是血浆复钙时间测试结果显示,细菌纳米纤维素/壳聚糖管的抗凝性略弱于细菌纳米纤维素管;③细胞相容性实验表明,猪髋动脉内皮细胞在细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管上均能稳定增殖生长,但是在细菌纳米纤维素/壳聚糖管上的增殖效果没有细菌纳米纤维素管明显。BACKGROUND： There are still no applicable vascular grafts for clinical replacement of the small-diameter blood vessels（〈 6 mm）.OBJECTIVE： To explore the possibility of bacterial nano-cellulose （BNC）/chitosan （CH） composite tubes assmall-diameter artificial blood vessels.METHODS： BNC/CH tubes were fabricated by introducing chitosan （0.5%, 1%, 2%） into BNC tubes. Physicochemicalproperties of BNC/CH tubes were analyzed including macro-morphology, microstructure, density, water-holding capacity,burst pressure, mechanical properties, blood compatibility and cytocompatibility.RESULTS AND CONCLUSION： The results indicated that, as compared with the BNC tubes, the water permeability ofBNC/CH tubes reduced, the density and water-holding capacity increased, as well as the axial and radial mechanicalperformance was enhanced, but ductility and elasticity of BNC/CH tubes were weakened. Regarding blood compatibility,hemolysis ratios of BNC and BNC/CH met the requirements of medical grade composites, while the plasmarecalcification time showed that anticoagulation of BNC/CH tubes was slightly weaker than that of the BNC tubes. Cellcompatibility experiments showed that porcine hip artery endothelial cells stably proliferated on the inner surface of bothBNC and BNC/CH tubes, but the proliferation was more obvious on the BNC surface than on the BNC/CH surface.

关 键 词：纤维素 壳聚糖 人工血管 组织工程 生物材料 纳米材料 细菌纳米纤维素 复合管状材料 血液相容性 细胞相容性 小口径人工血管 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]