80%赖氨酸硫酸盐与98%赖氨酸盐酸盐对生长中期草鱼生长性能、消化吸收能力和消化器官生长发育影响的比较研究  

A Comparative Study:Effects of 80% L-lysine·H_2SO_4 and 98% L-lysine·HCl on Growth Performance,Digestion and Absorption Capacities and Growth Development of Digestive Organs of Young Grass Carp(Ctenopharyngodon idella)

作　　者：胡凯[1,2] 苏玥宁[1] 冯琳 刘扬[1,3,4] 姜维丹 吴培[1,3,4] 姜俊 周小秋[1,3,4] HU Kai;SU Yuening;FENG Lin;LIU Yangl,;JIANG Weidan;WU Pei;JIANG Jun;ZHOU Xiaoqiu(Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;Department of Animal and Veterinary Science, Chengdu Agricultural College, Chengdu 611130, China;Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China)

机构地区：[1]四川农业大学动物营养研究所,成都611130 [2]成都农业科技职业学院畜牧兽医分院,成都611130 [3]鱼类营养与安全生产四川省高校重点实验室,成都611130 [4]动物抗病营养教育部重点实验室,成都611130

出　　处：《动物营养学报》2017年第12期4372-4385,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：基金项目:四川省重大科技成果转化示范(2015CC0011);四川省青年创新团队(2017TD0002)

摘　　要：本试验通过比较80%赖氨酸硫酸盐[80%L-Lys·H2SO4,简称80赖氨酸(80-Lys)]与98%赖氨酸盐酸盐[98%L-Lys·HCl,简称98赖氨酸(98-Lys)]对生长中期草鱼生长性能、消化吸收能力和消化器官生长发育的影响,探讨80-Lys和98-Lys在草鱼上的生物效价并确定以80-Lys为赖氨酸(Lys)添加形式时饲料中Lys的适宜含量。选择初始体重为275.80 g左右的健康草鱼540尾,随机分成6组(每组3个重复,每个重复30尾鱼),分别饲喂Lys含量为0.8%(基础饲料)、1.0%、1.2%、1.4%和1.6%的添加80-Lys的饲料及Lys含量为1.2%的添加98-Lys的饲料60 d。结果表明:与基础饲料相比,饲料中添加适宜水平的80-Lys使饲料Lys含量达到1.2%时可显著提高生长中期草鱼的增重率(W GR),特定生长率(SGR),采食量(FI),全肠脂肪酶、淀粉酶活力,肝胰脏谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力,前、中、后肠碱性磷酸酶(AKP)、肌酸激酶(CK)活力,肝体指数与肠体指数以及前、后肠皱襞高度(P<0.05),显著降低血清GOT和GPT活力(P<0.05),且80-Lys对上述指标的作用效果显著优于98-Lys(P<0.05);此外,还可显著提高生长中期草鱼的饲料效率(FE),全肠胰蛋白酶活力,前、中、后肠Na+,K+-ATP酶(Na+,K+-ATPase)和γ-谷胺酰转肽酶(γ-GT)活力,肠长与肠长指数以及中肠皱襞高度(P<0.05),但80-Lys对上述指标的作用效果与98-Lys差异不显著(P>0.05)。由此得出,与98-Lys相比,80-Lys能更有效地提高生长中期草鱼的消化吸收能力,进而促进其生长。以80-Lys为Lys添加形式,以SGR和FE为标识,生长中期草鱼(276~667 g)饲料中Lys的最适含量分别为1.31%(占饲料蛋白质的4.68%)和1.27%(占饲料蛋白质的4.54%)。This study aimed to compare the effects of 80% L-lysine · H2SO4 （80-1ysine） and 98% L-lysine · HC1 （98-1ysine） on growth performance, digestion and absorption capacities and growth development of diges- tive organs of young grass carp （ Ctenopharyngodon idella）, to explore the biological values of 98-1ysine and 98-1ysine, and to determine the optimum dietary lysine content using 80-1ysine as the supplemental form of ly- sine. A total of 540 grass carp with an initial body weight about 275.80 g were randomly divided into 6 groups with 3 replicates each and 30 fish per replicate. Fish in 6 groups were fed adding 80-1ysine diets containing 0.8% （basal diet）, 1.0%, 1.2%, 1.4% and 1.6% lysine and adding 98-1ysine diet containing 1.2% lysine for 60 d, respectively. The results showed as follows： compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-1ysine could significantly increase the weight gain rate （WGR）, specific growth rate （SGR）, feed intake （FI）, the activities of lipase, amylase in total intestine, the activities of glu- tamic oxalacetic transaminase （GOT） and glutamate pyruvate transaminase （GPT） in hepatopancreas, the ac- tivities of alkaline phosphatase （AKP） and creatine kinase （CK） in proximal intestine （ PI）, mid intestine （MI） and distal intestine （DI）, hepatosomatic index （HSI）, intestosomatic index （ISI） and the fold height of PI and DI （P〈0.05）, and significantly decrease the activities of GOT and GPT in serum （P〈0.05） ; the effects of 80-1ysine on these parameters were better than 98-1ysine （P〈0.05）. Moreover, compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-1ysine could significantly increase the feed efficiency （FE）, total intestine trypsin activity, the activities of Na＊, K＋-ATPase and γ glutamyl- transferase （~/-GT） in PI, MI and DI, intestinal length, relative gut length （RGL） and fold height in MI （

关 键 词：80%赖氨酸硫酸盐 98%赖氨酸盐酸盐 草鱼 生长性能 消化吸收能力 

分 类 号：S963[农业科学—水产养殖]