慢性乙型肝炎患者外周血IL-37的表达水平及意义  

Expression and Clinical Significance of Blood IL-37 in Patients with Chronic HBV

作　　者：黄太宏[1] 徐学静[1] 胡伟[1] 王森[1] 

机构地区：[1]南京大学医学院附属鼓楼医院检验科,南京210008

出　　处：《现代检验医学杂志》2017年第6期26-27,31,共3页Journal of Modern Laboratory Medicine

基　　金：国家自然基金青年基金资助(81601765)

摘　　要：目的研究慢性乙型肝炎(chronic hepatitis B,CHB)患者外周血血清中白细胞介素-37(IL-37)的表达水平,并探讨IL-37在CHB中的作用及意义。方法通过ELISA检测35例CHB患者及35例健康对照者血清中IL-37的含量;全自动生化仪及化学发光仪检测CHB患者血清中ALT,AST,HBsAg,HBeAg的含量,定量PCR及ELISA方法检测CHB患者血清中HBV DNA载量及细胞因子TNF-α,IL-10的表达水平,并分析IL-37含量分别与ALT,AST,HBsAg,HBeAg,HBV DNA,TNF-α及IL-10之间的相关性。结果 CHB患者组血清中IL-37的表达水平437.5±152.7pg/ml,高于对照组(63.0±2.1pg/ml),两组之间差异具有统计学意义(t=2.45,P=0.017)。Pearson相关性分析显示,IL-37的水平与AST,HBsAg,HBeAg,HBV DNA,TNF-α的水平不相关,而与ALT(r=0.406,P=0.019),IL-10(r=0.433,P=0.035)的含量呈正相关。结论 CHB患者血清中IL-37的水平高于健康对照,IL-37可能在肝损伤及乙肝慢性化形成中发挥重要作用。Objective To study the expression levels of IL-37 in serum in chronic HBV（CHB）patients,and investigate their clinical significance in CHB patients.Methods The expression levels of IL-37 in serum from 35 CHB patients and 35 health-y controls were quantified by ELISA.This study also tested ALT,AST,HBsAg,HBeAg,HBV DNA,TNF-αand IL-10 in the serum of CHB patients,and also analyzed the relationship between the levels of IL-37 and ALT,AST,HBsAg,HBeAg, HBV DNA,TNF-α,IL-10 levels.Results The expression levels of IL-37 was increased in CHB patients 437.5±152.7 pg/ml compared to healthy donors（63.0±2.1 pg/ml）.The difference between the two groups was statistically significant（t=2.45,P=0.017）.The levels of IL-37 was found to correlate with ALT（r=0.406,P=0.019）and IL-10（r=0.433,P=0.035）but not AST,HBsAg,HBeAg,HBV DNA and TNF-α.Conclusion This study reveals the levels of IL-37 in CHB patients was higher than healthy controls,and IL-37 may play an important role in liver damage and HBV chronic infection.

关 键 词：慢性乙型肝炎 白细胞介素-37 炎性因子 

分 类 号：R512.62[医药卫生—内科学] R392.11[医药卫生—临床医学]