颈动脉粥样硬化斑块性质与患者血清miRNA-126,miRNA-155水平的相关性研究  

Clinical Application of Serum miRNA-126,miRNA-155 Detection in Evaluation of Plaque Property in the Carotid Atherosclerotic Desease

作　　者：姜彦峰 李小侠[2] 陈安志 

机构地区：[1]咸阳市中心医院检验科,陕西咸阳712000 [2]陕西省人民医院检验科,西安710068 [3]石泉县中医医院检验科,陕西石泉725200

出　　处：《现代检验医学杂志》2017年第6期74-77,80,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血清miRNA-126,miRNA-155检测在评价颈动脉粥样硬化(carotid athero sclerosis,CAS)斑块性质中的临床应用价值。方法选择2015年5月~2017年5月在咸阳市中心医院和石泉县中医医院就诊的CAS患者75例作为研究对象,依据颈部增强CT检测结果分为易损斑块组(35例)和稳定斑块组(40例)。同时选取39例同期体检健康者作为对照组。采用实时逆转录-聚合酶链反应(RT-PCR)技术检测各组血清miRNA-126,miRNA-155表达水平。采用颈部增强CT检测各组颈动脉最大斑块厚度(MAPT)及内中膜厚度(IMT)。结果颈动脉MAPT及IMT在易损斑块组的检测结果为3.27±1.01mm,1.93±0.51mm;在稳定斑块组的检测结果为2.50±0.79mm,1.60±0.26mm。易损斑块组的颈动脉MAPT及IMT高于稳定斑块组,差异均有统计学意义(t=9.76,7.86,P<0.01)。血清miRNA-126和miRNA-155在易损斑块组中的表达量为0.22±0.06和0.87±0.18;在稳定斑块组的表达量为0.50±0.12和0.47±0.10;在对照组的表达量为0.90±0.19和0.19±0.05。稳定斑块组和易损斑块组血清miRNA-155表达水平显著高于对照组,而miRNA-126表达水平显著低于对照组,差异均有统计学意义(F=119.3,102.9,均P<0.01)。易损斑块组miRNA-155表达水平显著高于稳定斑块组,而miRNA-126表达水平显著低于稳定斑块组,差异均有统计学意义(F=119.3,102.9,P<0.01)。易损斑块组miRNA-126与miRNA-155表达水平呈负相关性(r=-0.912,P<0.01);在该组中miRNA-126表达水平与MAPT及IMT呈负相关(r=-0.913,-0.893,P<0.01),而miRNA-155表达水平与颈动脉两项指标则呈正相关(r=0.899,0.907,P<0.01)。结论血清miRNA-126,miRNA-155检测可应用于颈动脉粥样硬化斑块破裂的预测,并可能成为缺血性脑卒中事件发生的有效预警标志物。Objective To investigate the clinical application of serum miRNA-126,miRNA-155 detection in evaluation of plaque property in the carotid atherosclerotic（CAS）desease.Methods A total of 75 patients with the CAS from May 2015 to May 2017 in the Xianyang Central Hospital and Shiquan Country Chinese Traditional Medicine was chosen,consisted of 35 cases of vulvernable plaque group and 40 cases of stable plaque group.Meanwhile,39 cases of healthy physical examines at the same time were regarded as the control group.The expression levels of serum miRNA-126,miRNA-155 in the groups were detected using the real-time reverse transcription-polymerase chain reaction technique.The largest carotid artery plaque thickness（MAPT）and intima-media thickness（IMT）in the groups were measured using the cervical enhancement CT.Re-sults The results of MAPT and IMT were（3.27±1.01 mm,1.93±0.51 mm）in the vulvernable plaque group and（2.50 ±0.79 mm,1.60±0.26 mm）in the stable plaque group.The carotid artery largest plaque thickness and intima-media thick-ness was higher in the vulvernable plaque group than in the stable plaque group（t=9.76,7.86,P＆lt;0.01）,and there were significant differenes between the two groups.The expression levels of serum miRNA-126and miRNA-155 were（0.22 ± 0.06,0.87±0.18）in the vulvernable plaque group,（0.50±0.12,0.47±0.10）in the stable plaque group and（0.90±0.19, 0.19±0.05）in the control group.MiRNA-155 expression levels significantly increased in stable plaque group and vulvern-able plaque group compared with in the control group,which increased in the vulvernable plaque group compared with in the stable plaque group,and miRNA-126 expression levels markedly decreased,the differences were statistically significant（F=119.3,102.9,P〈0.01）.In the vulvernable plaque group,miRNA-126 expression negatively correlated with miRNA-155（r=0.912,P〈0.01）.miRNA-126 expression levels were inversely associated with the carotid artery largest plaque thickness and the intima-media

关 键 词：颈动脉粥样硬化 斑块破裂 颈动脉最大斑块厚度 颈动脉内中膜厚度 MIRNAS miRNA-126 miRNA-155 

分 类 号：R543.5[医药卫生—心血管疾病] R392.11[医药卫生—内科学]