西安地区妇科患者阴道分泌物支原体检测及耐药性分析  

Detection and Drug Resistance of Mycoplasma in Vaginal Secretions of Gynecological Patients in Xi'an

作　　者：解娟[1] 刘文康[1] 李博[1] 范云[1] 陈红男 李玲[1] 

机构地区：[1]陕西省人民医院检验科,西安710068

出　　处：《现代检验医学杂志》2017年第6期118-121,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金资助项目(NO.81272856)

摘　　要：目的了解妇科门诊患者阴道分泌物中解脲支原体(ureaplasma yrealyticum,Uu)和人型支原体(mycoplasma hominis,Mh))的感染及药物敏感情况。方法收集2015年1月~2016年6月妇科门诊患者阴道分泌物共1 800例,采用郑州安图绿科生物工程有限公司生产的支原体培养鉴定计数药敏试剂盒检测阴道分泌物中的Uu,Mh及药敏试验。结果Uu检出率(57.27%)明显高于Mh检出率(2.78%)(χ~2=33.69,P<0.001);31~35岁患者阴道分泌物中支原体的检出率最高(77.09%),而21~25岁患者受检样本中Uu检出率最高(65.83%),36~40岁患者受检样本中Mh检出率最高(9.09%),20岁以下患者中Uu并发Mh感染率最高(20.51%),各年龄组的受检样本中Uu,Mh以及并发感染率之间的差异均有统计学意义(χ~2=15.505~36.574,P<0.01);支原体药物总敏感排前3位的依次是交沙霉素、美满霉素和强力霉素,后3位分别是克林霉素、甲砜霉素和司帕沙星。Uu药物敏感率均高于Uu并发Mh,而Mh对红霉素、阿奇霉素、克拉霉素、罗红霉素和加替沙星的敏感率均低于Uu并发Mh。结论检测患者阴道分泌物中支原体及药敏可以为临床诊断和治疗提供实验室检查依据。Objective To explore the infection and drug sensitivity of ureaplasma urealyticum（Uu）and mycoplasma hominis （Mh）in vaginal secretion of gynecological outpatients.Methods The infection and drug sensitivity test of Uu and Mh in va-ginal secretion samples of 1 800 patients collected from January 2015 to April 2016 were detected with mycoplasma culture i-dentification and counting drug sensitivity kit produced by Zhengzhou Antu Luke Bioengineering Co.,Ltd.Results The positive rate of Uu（57.27%）was significantly higher than that of Mh（2.78%,χ2=33.69,P〈0.001）.The positive rate of mycoplasma was highest in the age group of 31~35（77.09%）,but that of Uu was highest in the age group of 21~25 （65.83%）and that of Mh in 36~40 years old group（9.09%）,in addition that of multiple infection by Uu and Mh was highest in less than 20 years old group（20.51%）.There were statistical difference for Un,Mh and co-infection by Un and Mh between age groups（χ2=15.505~36.574,P〈0.01）.The top three drugs sensitive for mycoplasma were josamycin, minocycline and doxycycline and that last three ones were clindamycin,thiamphenicol and sparfloxacin.The drug sensitive rates for 12 antibiotics against Uu were higher than those against co-infection by Uu and Mh,but those of erythromycin,gat-ifloxacin,azithromycin,clarithromycin and Luo Hongmei against Mh were lower than those against co-infecion of Uu and Mh.Conclusion The detection of mycoplasma and drug sensitivity in vaginal secretions provides the experimental basis for clinical diagnosis and treatment.

关 键 词：阴道分泌物 解脲支原体 人型支原体 药敏试验 

分 类 号：R711.3[医药卫生—妇产科学] R375.3[医药卫生—临床医学]