水貂TOll样受体5编码区基因的克隆及序列分析  

Molecular cloning and sequence analysis of mink Toll-like receptor 5 gene CDS

作　　者：姜合祥 蔡孜萌 秦晓冰[1] 单虎[1] JIANG Hexiang;CAI Zimeng;QIN Xiaobing;SHAN Hu(College of Animal Science ＆ Veterinary Medicine, Qingdao Agrieuhural University, Qingdao 266109, China)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109

出　　处：《畜牧与兽医》2017年第12期70-74,共5页Animal Husbandry & Veterinary Medicine

基　　金：科技部科技基础性工作专项项目(2012FY111000);山东省优秀中青年科学科研奖励基金(博士基金)(BS2011SW010);山东省自主创新及成果转化专项(2014ZZCX07105);青岛农业大学研究生创新计划项目(1215004)

摘　　要：为了解水貂Toll样受体5(TLR5)蛋白的结构特征和遗传演化关系,采用RT-PCR方法从水貂肺脏组织总RNA中扩增出TLR5基因的片段,经拼接获得全长编码区序列。序列全长2 577 bp,编码858个氨基酸,含172%的亮氨酸,并有一段20个氨基酸的信号肽序列。蛋白预测结果表明,该分子具有亲水性,由胞外区(具有LRR结构域)、跨膜区和胞内区(具有TIR结构域)组成,表现出典型的TLR家族结构特征;同源性分析结果显示,与蒙眼貂同源性最高,达97%以上,与海象、大熊猫和北极熊同源性80%以上,与其他物种同源性大都在70%以上。水貂TLR5基因序列的成功克隆为进一步研究其在水貂机体免疫应答中的作用奠定了基础。To investigate genetic revolution and structural characteristics of mink Toll-like receptor 5（TLR5）, RT-PCR was performed toamplify TLR5 gene fragments after total RNA was extracted from mink lung, and the whole coded sequence was obtained by splicing. Thegene of 2577 bp encodes 858 amino acid, in which 17 2% Leucines and signal peptides of 20 amino acids are included. Protein predictionshowed that the protein is hydrophilicity, consisting of extracellular, transmembrane and intracellular regions, which appears typicalstructural characteristics of TLR family. Sequence analysis indicated that TLR5 of mink has the highest homology with that of ferret（97% i-dentity）, 80% higher homology with that of walrus, panda and polar bear, and almost 70% homology with that of the other species. TLR5 ishighly conservative during the evolution according to a phylogenetic tree. This study will provide a basis for investigation of TLR5 role in theimmunologic mechanism and disease resistance in minks.

关 键 词：水貂 Toll受体5 基因克隆 序列分析 结构预测 

分 类 号：Q784[生物学—分子生物学]