咖啡鞣酸在单纯疱疹病毒感染的小胶质细胞中抗炎机制研究  

Anti-inflammatory effects of chlorogenic acid in HSV-1 infected microglia

作　　者：邓娜[1] DENG Na(Children＇s Center, Shiiyan People＇s Hospital, Shiyan 442000, Chin)

机构地区：[1]十堰市人民医院儿童医疗中心,湖北十堰442000

出　　处：《现代医学》2017年第11期1637-1641,共5页Modern Medical Journal

摘　　要：目的:研究咖啡鞣酸(chlorogenic acid,CGA)在单纯疱疹病毒-1(herpes simplex virus-1,HSV-1)感染的BV2小胶质细胞中的抗炎作用。方法:用HSV-1刺激的BV2小胶质细胞建立单纯疱疹病毒性脑炎(herpes simplex virus encephalitis,HSE)的细胞模型,然后用不同浓度梯度的CGA进行处理。通过MTT法测定细胞存活率。分别通过real-time PCR(RT-PCR)和Western blotting测定Toll样受体TLR2、TLR9 mRNA和蛋白质的表达。结果:HSV-1感染的BV2小胶质细胞经CGA处理后细胞存活率显著升高(P<0.05)。与正常组相比,HSV-1组TLR2、TLR9的mRNA和蛋白的水平显著升高(P<0.05),而CGA作用后显著降低HSV-1诱导的TLR2、TLR9表达(P<0.05)。结论:CGA通过降低TLR2、TLR9的表达抑制HSE中的炎症反应,可为HSE的抗炎治疗提供新的治疗策略。Objective：To investigate the role of chlorogenic acid（CGA） in herpes simplex virus（HSV）-1-induced responses in BV2 microglia.Methods：The cellular model was established with BV2 cells stimulated by HSV-1 and then treated with CGA at different concentrations.Cell viability was assayed by the MTT assay.mRNA expression of Toll-like receptor（TLR）-2 and TLR9 was assayed by real-time quantitative PCR,and the protein expression was assayed by Western blotting.Results：The cell survival rate was significantly improved after CGA treatment（P〈0.05）,and CGA prevented the increases in TLR2 and TLR9 following HSV-1 challenge in BV2 cells both at mRNA and protein levels.Compared with the normal group,the levels of mRNA and protein of TLR-2 and TLR-9 in HSV-1 group were significantly increased（P〈0.05）,while CGA treatment significantly reduced the expression of TLR-2 and TLR-9 induced by HSV-1（P〈0.05）.Conclusions：CGA inhibits the inflammatory reaction in HSE via the suppression of TLR2 and TLR9.CGA may provide a new anti-inflammatory strategy for HSE treatment.

关 键 词：咖啡鞣酸 炎症 TOLL样受体 单纯疱疹病毒-1 

分 类 号：R978.7[医药卫生—药品] R511[医药卫生—药学]