快速检测镉离子的免疫层析分析法的建立及应用  

A rapid immunochromatographic assay for the detection of Cd^(2+)

作　　者：王玉珍[1] 周静清 温雅琼[1] 刘海燕[3] 冯锋[1] 

机构地区：[1]山西大同大学化学与环境工程学院,山西大同037009 [2]山西大学化学化工学院,山西太原030006 [3]山西大同大学有机化学研究所,山西大同037009

出　　处：《化学研究与应用》2017年第12期1916-1920,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21375083)资助;山西省大同市科技攻关项目(2015025)资助;山西大同大学博士科研启动项目(2012-B-08)资助

摘　　要：在制备出镉离子单克隆抗体的基础上,首先运用柠檬酸钠还原法合成了胶体金,然后用胶体金标记抗体,得到检测探针;优化实验条件后,组装试纸条,样品中的镉与检测线上面的抗原竞争有限的金标抗体,通过观察检测线的颜色,确定检出限;最后检测方法的特异性和灵敏度,建立了一种快速检测水样中镉离子的竞争免疫层析试纸条方法。结果:方法的检出限为50ng·mL-1,交叉反应结果表明,该抗体除与汞离子有交叉反应外,与其他金属离子(Zn^(2+)、Cr^(3+)、Pb^(2+)、Cu^(2+)、Ni^(2+)、Al^(3+)、Co^(2+)、Fe^(3+)、Mg^(2+)、Ca^(2+)和Ba^(2+))均无交叉反应。湖水样品中镉的加标实验结果显示与传统的酶联免疫分析方法有很好的相关性。建立的胶体金免疫层析试纸条方法可以特异、快速地检测水样中的重金属镉离子,在10min左右可以得到结果,适合用于现场检测。In this work,based on the monoclonal antibody(mAb)which can specifically recognize individual Cd-EDTA,a competitive immunochromatographic assay(ICA)using colloidal gold– mAb probe for rapid and direct detection of Cd^(2+) in water samples was described.Cd^(2+) in standard/sample solution competed with the coating antigen immobilized on the test line for limited mAb on the immunoprobe.The assay for Cd^(2+) was completed within 10 min.The visual detection of limit(LOD)of the ICA for Cd^(2+) was 50 ng·mL^(-1).There was no cross-reactivity with other metal ions except Hg^(2+).Real water samples spiked with different concentrations of Cd^(2+) were measured by ICA and the results were highly consistent with those obtained from enzyme-linked immunosorbent assay(ELISA).It was demonstrated that the proposed ICA was an applicable method for rapid and direct detection of Cd^(2+) on-site.

关 键 词：镉离子 单克隆抗体 胶体金 免疫层析技术 

分 类 号：O652.1[理学—分析化学]