盐胁迫下棉花LTR-反转座子的转录激活及在耐盐相关基因发掘中的应用  

The identification of long terminal repeat retrotransposons(LTR-RTs)with transcription activity under salt stress and its application in screening the candidate genes related to salt-tolerant in cotton

作　　者：黄芳[1,2] 徐珍珍 孟珊[2] 刘静 汪保华[1] 沈新莲[2] 

机构地区：[1]南通大学生命科学学院,江苏南通226019 [2]江苏省农业科学院经济作物研究所/农业部长江下游棉花和油菜重点实验室,江苏南京210014

出　　处：《江苏农业学报》2017年第6期1220-1226,共7页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技项目(BK20150540);国家转基因生物新品种培育重大专项(2014ZX08005-004-002);江苏省农业科技自主创新基金项目[CX(14)5008]

摘　　要：LTR-反转座子是棉花基因组的主要组成部分,在基因组中通常呈现"静止"状态。但受到胁迫刺激时,部分反转座子的转录活性可被"激活",可能影响邻近基因的表达。本研究在盐胁迫下通过生物信息学手段,在旱地棉转录组数据库中检测到3 885个转录激活的候选LTR-反转座子;这些候选LTR-反转座子上下游5 kb内共有1 787个邻近基因,其中377个邻近基因在盐胁迫下差异表达,通过对377个基因进行功能注释发现,326个基因注释到GO数据库中,并且部分基因与棉花已报道过的耐盐抗旱基因同源。本研究结果将为棉花的耐盐分子机理解析提供一定的理论基础。Long terminal repeat retrotransposons （LTR-RTs） are the most abundant genomic components in cotton. Normally, LTR-RTs keep in a quiescent state in their host genomes. Using a variety of stresses,the transcription activities of some LTR-RTs may be greatly activated,and they probably influence the expression of their related genes,such as adja-cent genes. In this study,3 885 candidate LTR-RTs with transcription activities were detected under salt stress based on the transcriptome data of Gossypium aridum. In parallel,1 787 genes with distances of less than 5 kb to these 3 885 LTR-RTs were identified, and 377 genes were differentially expressed under salt stress. 326 genes were annotated to the gene ontology database, and some of them were homologous genes with salt-tolerant and drought-resistant genes which had been reported in cotton. This study will provide a basis for the cotton molecular mechanism of salt-tolerant.

关 键 词：棉花 盐胁迫 LTR-反转座子 转录活性 邻近基因 

分 类 号：S562[农业科学—作物学]