干旱胁迫下华北驼绒藜叶片蛋白质的差异表达分析  

Analysis of differentially expressed proteins in leaf of Ceratoides arborescens under drought stress

作　　者：青格乐[1] 陶克陶巴雅尔 恩和其木格 王玉芝[3] 

机构地区：[1]内蒙古农牧业科学院,内蒙古呼和浩特010031 [2]蒙古国农业大学,乌兰巴托210153 [3]内蒙古林业科学研究院,内蒙古呼和浩特010010

出　　处：《北方农业学报》2017年第6期24-27,共4页Journal of Northern Agriculture

基　　金：内蒙古自治区自然科学基金项目(2014MS0349);内蒙古农牧业科学院青年创新基金项目(2012QNJJM03)

摘　　要：为了探讨华北驼绒藜抗旱机理,利用蛋白质组学方法分析鉴定了华北驼绒藜叶片在干旱胁迫下的差异表达蛋白质,结果共检测到20个差异表达蛋白质点,并成功鉴定其中具有代表性的7个蛋白质点,经数据库搜索对比这些蛋白质分别为Ru Bis CO活化酶3个、光合作用蛋白1个、能量代谢相关蛋白2个、逆境胁迫响应蛋白1个。可以推测,干旱胁迫对华北驼绒藜的生长代谢造成不同程度影响,这时其体内抗旱响应蛋白发挥作用,通过保护光合作用系统、提高植物的光合能力、促进植物体内糖分的积累等途径起到提升抗旱能力的作用。In order to investigate the mechanism of drought resistance of Ceratoides arborescens,the differentially expressed proteins were identified by molecular biology technique under drought stress. The results showed that twenty differentially expressed protein spots were detected and seven representative spats of them were successfully identified,including three Ru Bis CO activase,one photosynthetic protein,two energy metabolism related proteins, and one adversity stress corresponding protein. It is speculated that drought stress has affects on the growth and metabolism of Ceratoides arborescens;as response to the drought stress, the drought-resistant proteins enhance the drought resistance of Ceratoides arborescens by protecting the photosynthesis system,improving the photosynthetic capacity of plants,and promoting the accumulation of sugar in plants.

关 键 词：华北驼绒藜 干旱胁迫 响应蛋白 鉴定 

分 类 号：S548[农业科学—作物学]