液相悬浮芯片技术用于婴幼儿ToRCH病原体抗体检测的评估  

Determinations of infant ToRCH specific antibodies by multiplex flow immunoassay

作　　者：陈黎[1] 韩碧芸 马展[1] 王洁 张泓[1] 

机构地区：[1]上海市儿童医院上海交通大学附属儿童医院检验科,上海200040

出　　处：《检验医学》2017年第12期1148-1151,共4页Laboratory Medicine

基　　金：上海交通大学医工交叉基金项目(YG2013MS75);上海市卫计委适宜应用技术推广项目(2013SY059)

摘　　要：目的评价液相悬浮芯片技术检测婴幼儿To RCH病原体[弓形虫(TOX)、风疹病毒(RUB)、人巨细胞病毒(CMV)、单纯疱疹病毒(HSV)]抗体与传统方法的一致性。方法使用液相悬浮芯片技术对130例患儿的To RCH病原体的IgM和IgG抗体进行测定,并与化学发光法进行比对研究,比对不一致的样本进一步采用酶联免疫吸附试验(ELISA)进行对比。结果除抗CMV IgG抗体外,液相悬浮芯片技术的测定结果与化学发光法的一致率均在80.00%以上,二者具有良好的一致性(K>0.4)。而液相悬浮芯片技术检测抗CMV IgG抗体与化学发光法和ELISA的一致率分别仅为45.37%和47.22%,该方法与传统方法(化学发光法和ELISA)缺乏一致性(K值分别为0.10和0.11)。结论液相悬浮芯片技术在测定TOX抗体(IgM和IgG)、RUB抗体(IgM和IgG)、CMV抗体(IgM)和HSV抗体(IgM和IgG)时具有良好的一致性,而在进行抗CMV IgG抗体的测定时与化学发光法和ELISA均有较大差异。Objective To evaluate the consistency of multiplex flow immunoassay and traditional methods in the determinations of infant ToRCH specific antibodies [toxoplasma（TOX）,rubella（RUB）,cytomegalovirus （CMV） and herpes simplex virus（HSV）].Methods ToRCH-IgM and ToRCH-IgG antibodies were determined by multiplex flow immunoassay in 130 infants,and the results were compared with those of chemiluminescence. The samples with inconsistent results were further determined by enzyme-linked immunosorbent assay（ELISA）. Results Except for CMV IgG antibody,multiplex flow immunoassay and chemiluminescence had good consistency,and the consistency rate was ＆gt;80.00%（〉0.4）. However,for CMV IgG antibody,the consistency rates of multiplex flow immunoassay with chemiluminescence and ELISA were 45.37% and 47.22%（K=0.10 and 0.11）.Conclusions There is a good consistency for TOX antibodies（IgM and IgG）,RUB antibodies（IgM and IgG）,CMV antibody（IgM）and HSV antibodies（IgM and IgG） between multiplex flow immunoassay and traditional methods（chemiluminescence and ELISA）,while there is difference for CMV antibody（IgG）between chemiluminescence and ELISA.

关 键 词：液相悬浮芯片 ToRCH病原体 婴幼儿 

分 类 号：R446.61[医药卫生—诊断学]